Analysis of tetracyclines in honey by high-performance liquid chromatography/tandem mass spectrometry

被引:50
作者
Khong, SP [1 ]
Hammel, YA [1 ]
Guy, PA [1 ]
机构
[1] Nestec Ltd, Nestle Res Ctr, CH-1000 Lausanne 26, Switzerland
关键词
D O I
10.1002/rcm.1814
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A confirmatory method coupling liquid chromatography to tandem mass spectrometry (LC/MS/MS) is described for the determination of tetracycline, oxytetracycline, doxycycline and chlortetracycline in honey. Demeclocycline, another tetracycline molecule not reported for its usage in honey, was used as internal standard to quantify the four analytes. The sample preparation entails a clean-up on an Oasis HLB solid-phase extraction cartridge and analyses were realised by LC/MS/MS in selected reaction monitoring mode. The stability of tetracyclines was checked under various storage conditions at -20, +4 and +20degreesC (both under dark and light exposures). Indeed, tetracyclines are not stable molecules and the epimerisation phenomenon was evaluated in this work. Appropriate correction factors of the MS/MS responses of each epimer were studied for each of the four tetracyclines to accurately quantify them. Moreover, the matrix effects encountered during the LC/MS/MS analyses were also studied in spiked experiments from blank honey samples of various geographical origins and different flower types. Copyright (C) 2005 John Wiley Sons, Ltd.
引用
收藏
页码:493 / 502
页数:10
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