Endogenous reverse transcription assays reveal high-level resistance to the triphosphate of (-)2'-dideoxy-3'-thiacytidine by mutated M184V human immunodeficiency virus type 1

被引：44

作者：

Quan, YD

Gu, ZX

Li, XG

Li, Z

Morrow, CD

Wainberg, MA

机构：

[1] MCGILL UNIV,DEPT MED,MONTREAL,PQ,CANADA

[2] MCGILL UNIV,DEPT MICROBIOL,MONTREAL,PQ H3A 2T5,CANADA

[3] UNIV ALABAMA,DEPT MICROBIOL,BIRMINGHAM,AL 35294

来源：

JOURNAL OF VIROLOGY | 1996年 / 70卷 / 08期

关键词：

D O I：

10.1128/JVI.70.8.5642-5645.1996

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Kinetic analysis showed that the K-i values and the K-i/K-m ratios for mutated, recombinant M184V human immunodeficiency virus type 1 reverse transcriptase (RT) for (-)2'-dideoxy-3'-thiacytidine triphosphate (3TCTP) were 35-fold higher than the equivalent values for wild-type RT but only about twice as high as the equivalent values for each of the triphosphates of ddC (ddCTP) and ddA (ddATP). Fully endogenous RT assays showed that viruses containing the M184V substitution were highly resistant to 3TCTP, with an increase in the 50% inhibitory concentration of 250-fold in comparison with wild-type recombinant virus.

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收藏

页码：5642 / 5645

页数：4

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