Isolation and characterization of a rice cDNA encoding the γ-subunit of translation elongation factor 1B (eEF1Bγ)

被引：8

作者：

Kidou, S ^{[1
]}

Tsukamoto, S ^{[1
]}

Kobayashi, S ^{[1
]}

Ejiri, S ^{[1
]}

机构：

[1] Iwate Univ, Fac Agr, Cryobiosyst Res Ctr, Morioka, Iwate 0208550, Japan

来源：

FEBS LETTERS | 1998年 / 434卷 / 03期

关键词：

elongation factor 1 gamma; eEF1B gamma; cDNA; translation; rice;

D O I：

10.1016/S0014-5793(98)01014-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We isolated a rice cDNA clone (refg) encoding the gamma-subunit of translation elongation factor 1B (eEF-1B gamma; the old designation was EF-1 gamma). The refg encodes an open reading frame of 419 amino acids which shows a similarity to the equivalent sequences from animals and yeast. Complex formation analysis, which showed the recombinant protein of refg (His-eEF1B gamma) and formed a complex,vith GST-eEF-1B beta, indicated that the refg encodes rice eEF1B gamma of the eEF1B alpha beta gamma complex. Expression analysis showed that refg mRNA is very abundant in suspension-cultured cells during the exponential phase of growth, A DNA blot analysis indicated that refg is located at a single locus in the rice genome. (C) 1998 Federation of European Biochemical Societies.

引用

页码：382 / 386

页数：5

共 19 条

[1] NUCLEOTIDE-SEQUENCE OF A TRYPANOSOMA-CRUZI CDNA-ENCODING A PROTEIN HOMOLOGOUS TO MAMMALIAN EF1-GAMMA [J].

BILLAUTMULOT, O ;

POMMIER, V ;

SCHONECK, R ;

PLUMASMARTY, B ;

TAIBI, A ;

LOYENS, M ;

CAPRON, A ;

OUAISSI, MA .

NUCLEIC ACIDS RESEARCH, 1993, 21 (16) :3901-3901

[2] MOLECULAR-CLONING OF XENOPUS ELONGATION FACTOR-1-GAMMA, MAJOR M-PHASE PROMOTING FACTOR SUBSTRATE [J].

CORMIER, P ;

OSBORNE, HB ;

MORALES, J ;

BASSEZ, T ;

POULHE, R ;

MAZABRAUD, A ;

MULNERLORILLON, O ;

BELLE, R .

NUCLEIC ACIDS RESEARCH, 1991, 19 (23) :6644-6644

[3] INTERACTIONS AMONG 4 SUBUNITS OF ELONGATION-FACTOR-1 FROM RICE EMBRYO [J].

EJIRI, SI ;

KAWAMURA, R ;

KATSUMATA, T .

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION, 1994, 1217 (03) :266-272

[4]

EJIRI SI, 1986, METHOD ENZYMOL, V118, P140

[5] CLONING AND GENETIC-CHARACTERIZATION OF A CALCIUM-BINDING AND PHOSPHOLIPID-BINDING PROTEIN FROM SACCHAROMYCES-CEREVISIAE THAT IS HOMOLOGOUS TO TRANSLATION ELONGATION FACTOR-1-GAMMA [J].

KAMBOURIS, NG ;

BURKE, DJ ;

CREUTZ, CE .

YEAST, 1993, 9 (02) :151-163

[6] Isolation, characterization and mRNA expression of four cDNAs encoding translation elongation factor 1A from rice (Oryza sativa L.) [J].

Kidou, S ;

Ejiri, S .

PLANT MOLECULAR BIOLOGY, 1998, 36 (01) :137-148

[7] EUKARYOTIC TRANSLATION ELONGATION-FACTOR 1-GAMMA CONTAINS A GLUTATHIONE TRANSFERASE DOMAIN - STUDY OF A DIVERSE, ANCIENT PROTEIN SUPERFAMILY USING MOTIF SEARCH AND STRUCTURAL MODELING [J].

KOONIN, EV ;

MUSHEGIAN, AR ;

TATUSOV, RL ;

ALTSCHUL, SF ;

BRYANT, SH ;

BORK, P ;

VALENCIA, A .

PROTEIN SCIENCE, 1994, 3 (11) :2045-2054

[8] PRIMARY STRUCTURE OF ELONGATION FACTOR-1-GAMMA FROM ARTEMIA [J].

MAESSEN, GDF ;

AMONS, R ;

ZEELEN, JP ;

MOLLER, W .

FEBS LETTERS, 1987, 223 (01) :181-186

[9] CLONING AND CHARACTERIZATION OF THE CDNA-ENCODING RICE ELONGATION-FACTOR 1-BETA [J].

MATSUMOTO, S ;

TERUI, Y ;

XI, SX ;

TAIRA, H ;

EJIRI, S .

FEBS LETTERS, 1994, 338 (01) :103-106

[10] CLONING AND SEQUENCING OF THE CDNA-ENCODING RICE ELONGATION FACTOR-1-BETA' [J].

MATSUMOTO, S ;

OIZUMI, N ;

TAIRA, H ;

EJIRI, S .

FEBS LETTERS, 1992, 311 (01) :46-48

← 1 2 →