CLONING AND CHARACTERIZATION OF THE CDNA-ENCODING RICE ELONGATION-FACTOR 1-BETA

被引：14

作者：

MATSUMOTO, S ^{[1
]}

TERUI, Y ^{[1
]}

XI, SX ^{[1
]}

TAIRA, H ^{[1
]}

EJIRI, S ^{[1
]}

机构：

[1] IWATE UNIV,FAC AGR,DEPT BIOSCI & TECHNOL,MORIOKA,IWATE 020,JAPAN

来源：

FEBS LETTERS | 1994年 / 338卷 / 01期

关键词：

CDNA CLONING; ELONGATION FACTOR 1-BETA; RICE; TRANSLATION;

D O I：

10.1016/0014-5793(94)80125-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have cloned and sequenced a cDNA coding for rice elongation factor 1 beta (EF-1 beta). The clone was 1420 bp long and contained an open reading frame coding for 229 amino acids. The overall identity between rice EF-1 beta and rice EF-1 beta' [Matsumoto, S., Oizumi, N., Taira, H. and Ejiri, S. (1992) FEBS Lett. 311, 46-48] is 60% at the amino acid sequence level; a higher percent identical residues (81%) were especially observed in the C-terminal region. Rice EF-1 beta has no conserved phosphorylation site for casein kinase II and no leucine zipper motif, although these motifs are well conserved in EF-1 delta (=beta in plants) subunits of animal EF-1.

引用

页码：103 / 106

页数：4

共 20 条

[1] ORGANIZATION AND NUCLEOTIDE-SEQUENCE OF A NEW RIBOSOMAL OPERON IN ESCHERICHIA-COLI CONTAINING THE GENES FOR RIBOSOMAL-PROTEIN S2 AND ELONGATION-FACTOR TS [J].

AN, G ;

BENDIAK, DS ;

MAMELAK, LA ;

FRIESEN, JD .

NUCLEIC ACIDS RESEARCH, 1981, 9 (16) :4163-4172

[2]

CHEN EY, 1985, NUCLEIC ACIDS RES, V4, P165

[3] ELONGATION FACTOR-I CONTAINS 2 HOMOLOGOUS GUANINE-NUCLEOTIDE EXCHANGE PROTEINS AS SHOWN FROM THE MOLECULAR-CLONING OF BETA AND DELTA SUBUNITS [J].

CORMIER, P ;

OSBORNE, HB ;

MORALES, J ;

BASSEZ, T ;

MINELLA, O ;

POULHE, R ;

BELLE, R ;

MULNERLORILLON, O .

NUCLEIC ACIDS RESEARCH, 1993, 21 (03) :743-743

[4] EFFECT OF CYCLIC-AMP AND CYCLIC-GMP ON THE AUTOPHOSPHORYLATION OF ELONGATION FACTOR-I FROM WHEAT EMBRYOS [J].

EJIRI, S ;

HONDA, H .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1985, 128 (01) :53-60

[5]

EJIRI S, 1989, INT S MOL ORG BIOL S, P238

[6]

EJIRI SI, 1986, METHOD ENZYMOL, V118, P140

[7] A TECHNIQUE FOR RADIOLABELING DNA RESTRICTION ENDONUCLEASE FRAGMENTS TO HIGH SPECIFIC ACTIVITY [J].

FEINBERG, AP ;

VOGELSTEIN, B .

ANALYTICAL BIOCHEMISTRY, 1983, 132 (01) :6-13

[8] CLONING AND CHARACTERIZATION OF THE ELONGATION-FACTOR EF-1-BETA HOMOLOG OF SACCHAROMYCES-CEREVISIAE - EF-1-BETA IS ESSENTIAL FOR GROWTH [J].

HIRAGA, K ;

SUZUKI, K ;

TSUCHIYA, E ;

MIYAKAWA, T .

FEBS LETTERS, 1993, 316 (02) :165-169

[9]

JANSSEN GMC, 1988, J BIOL CHEM, V263, P11063

[10] AN ANALYSIS OF 5'-NONCODING SEQUENCES FROM 699 VERTEBRATE MESSENGER-RNAS [J].

KOZAK, M .

NUCLEIC ACIDS RESEARCH, 1987, 15 (20) :8125-8148

← 1 2 →