CLONING AND CHARACTERIZATION OF THE ELONGATION-FACTOR EF-1-BETA HOMOLOG OF SACCHAROMYCES-CEREVISIAE - EF-1-BETA IS ESSENTIAL FOR GROWTH

被引：47

作者：

HIRAGA, K ^{[1
]}

SUZUKI, K ^{[1
]}

TSUCHIYA, E ^{[1
]}

MIYAKAWA, T ^{[1
]}

机构：

[1] HIROSHIMA UNIV,FAC ENGN,DEPT FERMENTAT TECHNOL,HIGASHI,HIROSHIMA 724,JAPAN

来源：

FEBS LETTERS | 1993年 / 316卷 / 02期

关键词：

ELONGATION FACTOR-1-BETA; CALMODULIN BINDING PROTEIN; GENE DISRUPTION; SACCHAROMYCES-CEREVISIAE;

D O I：

10.1016/0014-5793(93)81208-H

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A Saccharomyces cerevisiae cDNA homologue of the elongation factor EF-1beta was found among the clones obtained by immunoscreening of a yeast cDNA expression library with an antibody against calmodulin affinity-purified proteins. The cDNA encoded a protein of 206 amino acids which was highly homologous (about 70% homology) with Artemia salina and human EF-1beta. A protein with an apparent molecular mass of 33,000, significantly larger than that expected from the gene, was identified by Western blotting. Gene disruption experiments indicated that EF-1beta is essential for growth.

引用

页码：165 / 169

页数：5

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