Chitosan-g-poly(N-isopropylacrylamide) based nanogels for tumor extracellular targeting

被引：87

作者：

Duan, Cunxian ^{[1
]}

Zhang, Dianrui ^{[1
]}

Wang, Feihu ^{[1
]}

Zheng, Dandan ^{[1
]}

Jia, Lejiao ^{[1
]}

Feng, Feifei ^{[1
]}

Liu, Yue ^{[1
]}

Wang, Yancai ^{[1
]}

Tian, Keli ^{[2
]}

Wang, Fengshan ^{[3
]}

Zhang, Qiang ^{[4
]}

机构：

[1] Shandong Univ, Sch Pharmaceut Sci, Dept Pharmaceut, Jinan 250012, Peoples R China

[2] Shandong Univ, Sch Med, Inst Biochem & Mol Biol, Jinan 250012, Peoples R China

[3] Shandong Univ, Sch Pharmaceut Sci, Natl Glycoengn Res Ctr, Jinan 250012, Peoples R China

[4] Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100083, Peoples R China

来源：

INTERNATIONAL JOURNAL OF PHARMACEUTICS | 2011年 / 409卷 / 1-2期

关键词：

Nanogels; Chitosan; N-isopropylacrylamide; Tumor extracellular pH; Oridonin; DRUG-DELIVERY; IN-VITRO; N-ISOPROPYLACRYLAMIDE; PH; NANOPARTICLES; MICELLES; CHITOSAN; HYDROGELS; RELEASE; VIVO;

D O I：

10.1016/j.ijpharm.2011.02.050

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The principle objective of this research was to develop and characterize pH-responsive and biocompatible nanogels as a tumor-targeting drug delivery system. The nanogels were self-assembled from chitosan-based copolymers, chitosan-graft-poly(N-isopropylacrylamide) (CS-g-PNIPAm). The copolymers were synthesized via free radical copolymerization and characterized for their chemical structure by FT-IR and (1)H NMR. These copolymers could be efficiently loaded with oridonin (ORI) and the characteristics of ORI-loaded nanogels were evaluated. Drug release researches indicated that the ORI-loaded nanogels displayed pH-dependent release behaviors. Based on MTT assay and cellular morphological analysis, the anti-tumor activity of ORI-loaded nanogels was higher at pH 6.5 than at pH 7.4. In conclusion, the obtained nanogels appeared to be of great promise in tumor extracellular pH targeting for ORI. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：252 / 259

页数：8

共 24 条

[1] Temperature-sensitive chitosan-poly(N-isopropylacrylamide) interpenetrated networks with enhanced loading capacity and controlled release properties [J].

Alvarez-Lorenzo, C ;

Concheiro, A ;

Dubovik, AS ;

Grinberg, NV ;

Burova, TV ;

Grinberg, VY .

JOURNAL OF CONTROLLED RELEASE, 2005, 102 (03) :629-641

[2] Synthesis and characterization of thermo- and pH-sensitive hydrogels based on Chitosan-grafted N-isopropylacrylamide via γ-radiation [J].

Cai, H ;

Zhang, ZP ;

Sun, PC ;

He, BL ;

Zhu, XX .

RADIATION PHYSICS AND CHEMISTRY, 2005, 74 (01) :26-30

[3] pH-triggered injectable hydrogels prepared from aqueous N-palmitoyl chitosan: In vitro characteristics and in vivo biocompatibility [J].

Chiu, Ya-Ling ;

Chen, Sung-Ching ;

Su, Chun-Jen ;

Hsiao, Chun-Wen ;

Chen, Yu-Ming ;

Chen, Hsin-Lung ;

Sung, Hsing-Wen .

BIOMATERIALS, 2009, 30 (28) :4877-4888

[4] Inner core segment design for drug delivery control of thermo-responsive polymeric micelles [J].

Chung, JE ;

Yokoyama, M ;

Okano, T .

JOURNAL OF CONTROLLED RELEASE, 2000, 65 (1-2) :93-103

[5] Synthesis and characterization of AB-crosslinked graft copolymers based on maleilated chitosan and N-isopropylacrylamide [J].

Don, TM ;

Chen, HR .

CARBOHYDRATE POLYMERS, 2005, 61 (03) :334-347

[6] Novel super pH-sensitive nanoparticles responsive to tumor extracellular pH [J].

Fan, Li ;

Wu, Hong ;

Zhang, Hui ;

Li, Fel ;

Yang, Tie-hong ;

Gu, Chun-hu ;

Yang, Qian .

CARBOHYDRATE POLYMERS, 2008, 73 (03) :390-400

[7] Tumor specific low pH environments enhance the cytotoxicity of lovastatin and cantharidin [J].

Fukamachi, Toshihiko ;

Chiba, Yoshie ;

Wang, Xin ;

Saito, Hiromi ;

Tagawa, Masatoshi ;

Kobayashi, Hiroshi .

CANCER LETTERS, 2010, 297 (02) :182-189

[8] Injectable pH- and Temperature-Responsive Poly(N-isopropylacrylamide-co-propylacrylic acid) Copolymers for Delivery of Angiogenic Growth Factors [J].

Garbern, Jessica C. ;

Hoffman, Allan S. ;

Stayton, Patrick S. .

BIOMACROMOLECULES, 2010, 11 (07) :1833-1839

[9] One-pot synthesis of pH- and thermo-sensitive chitosan-based nanoparticles by the polymerization of acrylic acid/chitosan with macro-RAFT agent [J].

Jiang, Jing ;

Hua, Daoben ;

Tang, Jing .

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2010, 46 (01) :126-130

[10]

LANGERMAN L, 1990, ANESTH ANALG, V71, P529

← 1 2 3 →