A quantitative and specific method for measuring transcript levels of highly homologous genes

被引：7

作者：

Lombardo, AJ

Brown, GB

机构：

[1] Department of Psychiatry, University of Alabama at Birmingham, Birmingham

来源：

NUCLEIC ACIDS RESEARCH | 1996年 / 24卷 / 23期

关键词：

D O I：

10.1093/nar/24.23.4812

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Because of their high nucleotide sequence homology, the specific detection of mRNA transcripts of individual members of a gene family presents certain problems. Here we apply and defend the single nucleotide primer extension (SNuPE) as a simple, specific, and highly quantitative assay for this purpose. The method distinguishes regions of the brain sodium channel gene family that vary by as little as a single nucleotide. The technique has been modified to include an intersample normalizer, and adaptation of the SNuPE assay to other gene families of interest to neuroscientists should be easy to achieve.

引用

收藏

页码：4812 / 4816

页数：5

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