The urokinase-type plasminogen activator receptor is not required for skeletal muscle inflammation or regeneration

被引:15
作者
Bryer, Scott C. [1 ]
Koh, Timothy J. [1 ]
机构
[1] Univ Illinois, Dept Movement Sci, Chicago, IL 60612 USA
关键词
muscle repair; macrophage; chemotaxis;
D O I
10.1152/ajpregu.00132.2007
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
The hypothesis of this study was the urokinase-type plasminogen activator receptor ( uPAR) is required for accumulation of inflammatory cells in injured skeletal muscle and for efficient muscle regeneration. Expression of uPAR was elevated at 1 and 3 days after cardiotoxin-induced muscle injury in wild-type mice before returning to baseline levels. Neutrophil accumulation peaked 1 day postinjury in muscle from both wild-type ( WT) and uPAR null mice, while macrophage accumulation peaked between 3 and 5 days postinjury, with no differences between strains. Histological analyses confirmed efficient muscle regeneration in both wild-type and uPAR null mice, with no difference between strains in the formation or growth of regenerating fibers, or recovery of normal morphology. Furthermore, in vitro experiments demonstrated that chemotaxis is not different between WT and uPAR null macrophages. Finally, fusion of cultured satellite cells into multinucleated myotubes was not different between cells isolated from WT and uPAR null mice. These results demonstrate that uPAR is not required for the accumulation of inflammatory cells or the regeneration of skeletal muscle following injury, suggesting uPA can act independently of uPAR to regulate events critical for muscle regeneration.
引用
收藏
页码:R1152 / R1158
页数:7
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