YPL.db2:: the Yeast Protein Localization database, version 2.0

被引:26
作者
Kals, M
Natter, K
Thallinger, GG
Trajanoski, Z
Kohlwein, SD
机构
[1] Graz Univ, Inst Mol Biosci, Biomembrane Res Ctr, SFB, A-8010 Graz, Austria
[2] Graz Univ Technol, Inst Genom & Bioinformat, A-8010 Graz, Austria
关键词
green fluorescent protein; subcellular localization; image database; microscopy;
D O I
10.1002/yea.1204
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The Yeast Protein Localization database (YPL.db(2)) is an archive of microscopic image data of protein localization patterns in the yeast Saccharomyces cerevisiae. The current version of YPL.db(2) harbours 500 sets of image data derived from high-resolution microscopic analyses of proteins tagged with the green fluorescent protein (GFP). Major functional improvements in YPL.db(2) over a previous release are a web-based experiment and image submission interface, facilitating standardized data entry by remote users through the Internet. The image display page provides image gallery and image scrolling features. In addition, fluorescence and transmission images can be superimposed, allowing image fading for precise correlation of the protein's localization in the cellular context. The reference structure database displaying 'prototypic' localization patterns was extended, and a feature to display and manipulate 3D-image datasets, using a freely available VRML plug-in, was included. Access to the Yeast Protein Localization database version 2.0 (YPL.db(2)) is available through http://YPL.uni-graz.at. Copyright (C) 2005 John Wiley Sons, Ltd.
引用
收藏
页码:213 / 218
页数:6
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