Somatic hybrid plantlets regeneration between Citrus and its wild relative, Murraya paniculata via protoplast electrofusion

被引：32

作者：

Guo, WW ^{[1
]}

Deng, XX ^{[1
]}

机构：

[1] Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Peoples R China

来源：

PLANT CELL REPORTS | 1998年 / 18卷 / 3-4期

基金：

中国国家自然科学基金;

关键词：

somatic hybridization; RAPD; Citrus huanglongbin; sexual and graft incompatibility; aurantioideae;

D O I：

10.1007/s002990050575

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Protoplasts isolated from 'Page' tangelo (Minneola tangelo x clementine) cell suspension cultures were electric ally fused with mesophyll protoplasts of orange jessamine [Murraya paniculata (L.) Jack]. Shoots were regenerated after 6 - 10 months of culture, but they were extremely recalcitrant to producing roots in root-induction medium. Complete plantlets were formed via micrografting. Chromosome counting of shoot tips revealed they were tetraploids (2n = 4x = 36). Glutamateoxaloacetate transaminase isozyme and randomly amplified polymorphic DNA analysis confirmed their hybridity. Orange jessamine is immune to citrus huanglongbin, a severe disease of citrus, but sexual incompatibility and limited graft compatibility exist between Citrus and orange jessamine. The cell fusion technique may make it possible to transfer the huanglongbin resistance trait from orange jessamine to Citrus.

引用

页码：297 / 300

页数：4