Development of a Highly Sensitive Fluorescence Probe for Hydrogen Peroxide

被引：349

作者：

Abo, Masahiro ^{[1
,3
]}

Urano, Yasuteru ^{[2
]}

Hanaoka, Kenjiro ^{[1
,3
]}

Terai, Takuya ^{[1
,3
]}

Komatsu, Toru ^{[1
,3
]}

Nagano, Tetsuo ^{[1
,3
]}

机构：

[1] Univ Tokyo, Grad Sch Pharmaceut Sci, Bunkyo Ku, Tokyo 1130033, Japan

[2] Univ Tokyo, Grad Sch Med, Bunkyo Ku, Tokyo 1130033, Japan

[3] JST, CREST, Chiyoda Ku, Tokyo 1020075, Japan

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2011年 / 133卷 / 27期

关键词：

EPIDERMAL-GROWTH-FACTOR; LIVING CELLS; CHROMOPHORE; ACTIVATION; MECHANISMS; GENERATION; INDICATOR; DESIGN; ROS;

D O I：

10.1021/ja203521e

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Hydrogen peroxide is believed to play a role in cellular signal transduction by reversible oxidation of proteins. Here, we report the design and synthesis of a novel fluorescence probe for hydrogen peroxide, utilizing a photoinduced electron transfer strategy based on benzil chemistry to control the fluorescence. The practical value of this highly sensitive and selective fluorescence probe, NBzF, was confirmed by its application to imaging of hydrogen peroxide generation in live RAW 264.7 macrophages. NBzF was also employed for live cell imaging of hydrogen peroxide generated as a signaling molecule in A431 human epidermoid carcinoma cells.

引用

页码：10629 / 10637

页数：9

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