Antiherpetic activity of a sulfated polysaccharide from Agaricus brasiliensis mycelia

被引：85

作者：

Gomes de Sousa Cardozo, Francielle Tramontini ^{[2
]}

Camelini, Carla Maisa ^{[2
]}

Mascarello, Alessandra ^{[3
]}

Rossi, Marcio Jose ^{[2
]}

Nunes, Ricardo Jose ^{[3
]}

Monte Barardi, Celia Regina ^{[2
]}

de Mendonca, Margarida Matos ^{[2
]}

Oliveira Simoes, Claudia Maria ^{[1
]}

机构：

[1] Univ Fed Santa Catarina, CCS, Lab Virol Aplicada, Dept Ciencias Farmaceut, BR-88040900 Florianopolis, SC, Brazil

[2] Univ Fed Santa Catarina, Dept Microbiol Imunol & Parasitol, BR-88040900 Florianopolis, SC, Brazil

[3] Univ Fed Santa Catarina, Dept Quim, BR-88040900 Florianopolis, SC, Brazil

来源：

ANTIVIRAL RESEARCH | 2011年 / 92卷 / 01期

关键词：

Agaricus brasiliensis; Sulfated polysaccharide; HSV-1; HSV-2; Antiherpes activity; Mechanism of action; ANTIVIRAL ACTIVITY; BLAZEI MURRILL; BETA-GLUCANS; VERO CELLS; VIRUS; MODE; REPLICATION; INHIBITION; ANTAGONISM; GALACTAN;

D O I：

10.1016/j.antiviral.2011.07.009

中图分类号：

R9 [药学];

学科分类号：

100702 [药剂学];

摘要：

Sulfated polysaccharides are good candidates for drug discovery in the treatment of herpetic infections. Agaricus brasiliensis (syn A. subrufescens, A. blazei) is a Basidiomycete fungus native to the Atlantic forest region of Southeastern Brazil. Herein we report the chemical modification of a polysaccharide extracted from A. brasiliensis mycelia to obtain its sulfated derivative (MI-S), which presented a promising inhibitory activity against HSV-1 [KOS and 29R (acyclovir-resistant) strains] and HSV-2 strain 333, with selectivity indices (SI = CC50/IC50) higher than 439, 208, and 562, respectively. The mechanisms underlying this inhibitory activity were scrutinized by plaque assay with different methodological strategies. MI-S had no virucidal effects, but inhibited HSV-1 and HSV-2 attachment, penetration, and cell-to-cell spread, as well as reducing the expression of HSV-1 ICP27, UL42, gB, and gD proteins. MI-S also presented synergistic antiviral effect with acyclovir. These results suggest that MI-S presents multiple modes of anti-HSV action. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：108 / 114

页数：7

共 36 条

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