The Effects of Nanofiber Topography on Astrocyte Behavior and Gene Silencing Efficiency

被引：20

作者：

Cao, Haoqing ^{[1
]}

Marcy, Guillaume ^{[2
]}

Lay, Eyleen

Goh, Keow ^{[2
]}

Wang, Feng ^{[3
,4
]}

Wang, Jun ^{[3
,4
]}

Chew, Sing Yian ^{[1
]}

机构：

[1] Nanyang Technol Univ, Sch Chem & Biomed Engn, Singapore 637459, Singapore

[2] DUKE NUS Grad Med Sch, Singapore 169857, Singapore

[3] Univ Sci & Technol China, Hefei Natl Lab Phys Sci Microscale, Hefei 230027, Anhui, Peoples R China

[4] Univ Sci & Technol China, Sch Life Sci, Hefei 230027, Anhui, Peoples R China

来源：

MACROMOLECULAR BIOSCIENCE | 2012年 / 12卷 / 05期

关键词：

electrospinning; fibers; films; neural tissue engineering; siRNA; POLY-EPSILON-CAPROLACTONE; SPINAL-CORD-INJURY; GLIAL SCAR; REACTIVE ASTROCYTES; NERVE REGENERATION; BLOCK-COPOLYMERS; SIRNA DELIVERY; DNA DELIVERY; AXON GROWTH; REPAIR;

D O I：

10.1002/mabi.201100436

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Astrocyte-nanofiber interactions are studied by culturing primary rat cortical astrocytes on poly[caprolactone-co-(ethyl ethylene phosphate)] electrospun nanofibers and solvent-cast films (two-dimensional control). The results indicate that nanofiber topography significantly suppresses astrocyte proliferation and enhances apoptosis, without altering cellular activation as compared to films. Moreover, nanofiber topography enhances gene-silencing efficiency in astrocytes. The results suggest that nanofibers may serve as potential substrates for nerve regeneration by suppressing astrocyte growth and may further facilitate the use of gene-silencing to enhance CNS regeneration.

引用

页码：666 / 674

页数：9

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