Single-molecule studies of DNA and DNA-protein interactions

被引：11

作者：

Feingold, M ^{[1
]}

机构：

[1] Ben Gurion Univ Negev, Dept Phys, IL-84105 Beer Sheva, Israel

来源：

PHYSICA E | 2001年 / 9卷 / 03期

关键词：

single-molecule biophysics; DNA-protein interactions; optical tweezers;

D O I：

10.1016/S1386-9477(00)00270-8

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

Two experiments are described that examine the properties of a single DNA molecule. The experimental system is based on optical tweezers and on a protocol that allows to attach one end of the DNA molecule to the bottom of the sample and the other to a bead of micron size. First, the reaction of the RecA protein with DNA is monitored in a particular DNA molecule. This is made possible by the fact that the DNA is locally extended by a factor of 1.5 whenever a RecA molecule binds. Second, we monitor the dynamics of a DNA molecule as it relaxes from a fully stretched state to the coil configuration. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

收藏

页码：616 / 620

页数：5

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