Mechanical separation of the complementary strands of DNA

被引：368

作者：

EssevazRoulet, B ^{[1
]}

Bockelmann, U ^{[1
]}

Heslot, F ^{[1
]}

机构：

[1] ECOLE NORMALE SUPER, URA 1437, PHYS MAT CONDENSEE LAB, F-75005 PARIS, FRANCE

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1997年 / 94卷 / 22期

关键词：

D O I：

10.1073/pnas.94.22.11935

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We describe the mechanical separation of the two complementary strands of a single molecule of bacteriophage lambda DNA. The 3' and 5' extremities on one end of the molecule are pulled progressively apart, and this leads to the opening of the double helix. The typical forces along the opening are in the range of 10-15 pN. The separation force signal is shown to be related to the local GC vs. AT content along the molecule. Variations of this content on a typical scale of 100-500 bases are presently detected.

引用

页码：11935 / 11940

页数：6

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