Evolution of cell-based reagent provision

被引：24

作者：

Cawkill, Darren ^{[1
]}

Eaglestone, Simon S. ^{[1
]}

机构：

[1] Pfizer Global Res & Dev, Sandwich CT13 9NJ, Kent, England

来源：

DRUG DISCOVERY TODAY | 2007年 / 12卷 / 19-20期

关键词：

D O I：

10.1016/j.drudis.2007.08.014

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Cell-based screening is now part of all stages of drug discovery, and therefore, cell supply is a rate-limiting step. Reagent provision groups have responded. by exploiting automation and new concepts such as frozen cells to ease the constraint and increase quality and flexibility of cell supply. With increasing numbers of projects to support, reagent development is now perceived as a new bottleneck. In this review, we describe a new operating model that has emerged at Pfizer UK addressing reagent development and cell supply issues without growing headcount, by complementing the application of internal expertise with use of contract research organisations.

引用

页码：820 / 825

页数：6

共 32 条

[1] BacMam recombinant Baculoviruses in G protein-coupled receptor drug discovery [J].

Ames, RS ;

Fornwald, JA ;

Nuthulaganti, P ;

Trill, JJ ;

Foley, JJ ;

Buckley, PT ;

Kost, TA ;

Wu, ZN ;

Romanos, MA .

RECEPTORS & CHANNELS, 2004, 10 (3-4) :99-107

[2] Dynamic and label-free cell-based assays using the real-time cell electronic sensing system [J].

Atienza, Josephine M. ;

Yu, Naichen ;

Kirstein, Shelli L. ;

Xi, Biao ;

Wang, Xiaobo ;

Xu, Xiao ;

Abassi, Yama A. .

ASSAY AND DRUG DEVELOPMENT TECHNOLOGIES, 2006, 4 (05) :597-607

[3]

BURKE JF, 2006, BIOPROCESS INT, V4, P48

[4] The selection of high-producing cell lines using flow cytornetry and cell sorting [J].

Carroll, S ;

Al-Rubeai, M .

EXPERT OPINION ON BIOLOGICAL THERAPY, 2004, 4 (11) :1821-1829

[5] Safety testing for replication-competent retrovirus associated with gibbon ape leukemia virus-pseudotyped retroviral vectors [J].

Chen, J ;

Reeves, L ;

Cornetta, K .

HUMAN GENE THERAPY, 2001, 12 (01) :61-70

[6] Cellular dielectric spectroscopy: A powerful new approach to label-free cellular analysis [J].

Ciambrone, GJ ;

Liu, VF ;

Lin, DC ;

McGuinness, RP ;

Leung, GK ;

Pitchford, S .

JOURNAL OF BIOMOLECULAR SCREENING, 2004, 9 (06) :467-480

[7] Outsourcing lead optimisation - the quiet revolution [J].

Clark, DE ;

Newton, CG .

DRUG DISCOVERY TODAY, 2004, 9 (11) :492-500

[8] Rapid cryopreservation of five mammalian and one mosquito cell line at -80°C while attached to flasks in a serum free cryopreservative [J].

Corsini, J ;

Mann, E .

BIOLOGICAL PROCEDURES ONLINE, 2005, 7 (1) :26-30

[9] Conditionally immortalized cell lines as model systems for high-throughput biology in drug discovery [J].

Daniele, N ;

Halse, R ;

Grinyo, E ;

Yeaman, SJ ;

Shepherd, PR .

BIOCHEMICAL SOCIETY TRANSACTIONS, 2002, 30 :800-802

[10] Components of successful lead generation [J].

Davis, AM ;

Keeling, DJ ;

Steele, J ;

Tomkinson, NP ;

Tinker, AC .

CURRENT TOPICS IN MEDICINAL CHEMISTRY, 2005, 5 (04) :421-439

← 1 2 3 4 →