Dynamic and label-free cell-based assays using the real-time cell electronic sensing system

被引：137

作者：

Atienza, Josephine M. ^{[1
]}

Yu, Naichen ^{[1
]}

Kirstein, Shelli L. ^{[1
]}

Xi, Biao ^{[1
]}

Wang, Xiaobo ^{[1
]}

Xu, Xiao ^{[1
]}

Abassi, Yama A. ^{[1
]}

机构：

[1] ACEA Biosci Inc, San Diego, CA 92121 USA

来源：

ASSAY AND DRUG DEVELOPMENT TECHNOLOGIES | 2006年 / 4卷 / 05期

关键词：

D O I：

10.1089/adt.2006.4.597

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Cell-based assays have become an integral part of the preclinical drug development process. Recently, noninvasive label-free cell-based assay technologies have taken center stage, offering important and distinct advantages over and in addition to traditional label-based endpoint assays. Dynamic monitoring of live cells, the preclusion of label, and kinetics are some of the fundamental features of cell-based label-free technologies. In this article we will discuss the real-time cell electronic sensing (RT-CES™, ACEA Biosciences Inc., San Diego, CA) system and some of its key applications for cell-based assays such as cell proliferation and cytotoxicity, functional assays for receptor-ligand analysis, cell adhesion and spreading assays, dynamic monitoring of endothelial barrier function, and dynamic monitoring of cell migration and invasion. Also, where appropriate we will briefly discuss other label-free technologies in an application-specific manner. © Mary Ann Liebert, Inc.

引用

页码：597 / 607

页数：11

共 48 条

[1] Label-free, real-time monitoring of IgE-mediated mast cell activation on microelectronic cell sensor arrays [J].