Isolation and expression analysis of gibberellin 20-oxidase homologous gene in apple

被引:22
作者
Kusaba, S [1 ]
Honda, C [1 ]
Kano-Murakami, Y [1 ]
机构
[1] Natl Inst Fruit Tree Sci, Dept Pomol, Tsukuba, Ibaraki 3058605, Japan
关键词
Malus x domestica; gibberellin; biosynthesis; GA; 20-oxidase; immature seed;
D O I
10.1093/jexbot/52.355.375
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
To characterize the gibberellin (GA) 20-oxidase gene in apple. the genomic and cDNA clone from 'Fuji' apple (accession no, ABO37114) was isolated, The deduced amino acid sequence of this cDNA showed 71% and 669 a identity to those of GA 20-oxidase cloned from French bean and Arabidopsis, respectively. The transcript of this gene was detected mainly in immature seeds between 1-3 months after full bloom, These results suggested that this apple GA 20-oxidase gene might be involved in GA biosynthesis in developing apple seed.
引用
收藏
页码:375 / 376
页数:2
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