CLA production from ricinoleic acid by lactic acid bacteria

被引:46
作者
Ando, A [1 ]
Ogawa, J [1 ]
Kishino, S [1 ]
Shimizu, S [1 ]
机构
[1] Kyoto Univ, Grad Sch Agr, Div Appl Life Sci, Sakyo Ku, Kyoto 6068502, Japan
基金
日本学术振兴会;
关键词
CLA; castor oil; conjugated linoleic acid; lactic acid bacteria; Lactobacillus plantarum; ricinoleic acid;
D O I
10.1007/s11746-003-0790-1
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
The ability to produce CLA from ricinoleic acid is widely distributed in lactic acid bacteria. Washed cells of Lactobacillus plantarum JCM 1551 were selected as a potential catalyst for CLA production from ricinoleic acid. Cells cultivated in medium supplemented with a mixture of a-linolenic acid and linoleic acid showed enhanced CLA productivity. Under optimal reaction conditions, with the free acid form of ricinoleic acid as the substrate and washed cells of L. plantarum as the catalyst, 2.4 mg/mL CLA was produced from 3.4 mg/mL ricinoleic acid in 90 h, with a molar yield with respect to ricinoleic acid of 71%. The CLA produced, which was obtained in the FFA form, consisted of a mixture of two CLA isomers, cis-9,trans-11-octadecadienoic acid (21% of total CLA) and trans-9,trans-11-octadecadienoic acid (79% of total CLA), and accounted for 72% of the total FA obtained.
引用
收藏
页码:889 / 894
页数:6
相关论文
共 19 条
[1]  
BELURY MA, 1995, NUTR REV, V53, P83
[2]  
BLIGH EG, 1959, CAN J BIOCHEM PHYS, V37, P911
[3]   ANTICARCINOGENS FROM FRIED GROUND-BEEF - HEAT-ALTERED DERIVATIVES OF LINOLEIC-ACID [J].
HA, YL ;
GRIMM, NK ;
PARIZA, MW .
CARCINOGENESIS, 1987, 8 (12) :1881-1887
[4]  
HA YL, 1990, CANCER RES, V50, P1097
[5]   Lipase-catalyzed fractionation of conjugated linoleic acid isomers [J].
Haas, MJ ;
Kramer, JKG ;
McNeill, G ;
Scott, K ;
Foglia, TA ;
Sehat, N ;
Fritsche, J ;
Mossoba, MM ;
Yurawecz, MP .
LIPIDS, 1999, 34 (09) :979-987
[6]  
IP C, 1991, CANCER RES, V51, P6118
[7]   FATTY-ACID DESATURATION-DEFECTIVE MUTANTS OF AN ARACHIDONIC-ACID-PRODUCING FUNGUS, MORTIERELLA-ALPINA 1S-4 [J].
JAREONKITMONGKOL, S ;
SHIMIZU, S ;
YAMADA, H .
JOURNAL OF GENERAL MICROBIOLOGY, 1992, 138 :997-1002
[8]  
KEPLER CR, 1966, J BIOL CHEM, V241, P1350
[9]   Structural analysis of conjugated linoleic acid produced by Lactobacillus plantarum, and factors affecting isomer production [J].
Kishino, S ;
Ogawa, J ;
Ando, A ;
Iwashita, T ;
Fujita, T ;
Kawashima, H ;
Shimizu, S .
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 2003, 67 (01) :179-182
[10]   Conjugated linoleic acid production from linoleic acid by lactic acid bacteria [J].
Kishino, S ;
Ogawa, J ;
Omura, Y ;
Matsumura, K ;
Shimizu, S .
JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 2002, 79 (02) :159-163