Human monoclonal antibodies to West Nile virus identify epitopes on the prM protein

被引：14

作者：

Calvert, Amanda E. ^{[1
]}

Kalantarov, Gavreel F. ^{[2
]}

Chang, Gwong-Jen J. ^{[1
]}

Trakht, Ilya ^{[2
]}

Blair, Carol D. ^{[3
]}

Roehrig, John T. ^{[1
]}

机构：

[1] Ctr Dis Control & Prevent, Arboviral Dis Branch, Div Vector Borne Infect Dis, Publ Hlth Serv,US Dept Hlth & Human Serv, Ft Collins, CO 80521 USA

[2] Columbia Univ, Coll Phys & Surg, Dept Med, New York, NY 10032 USA

[3] Colorado State Univ, Dept Microbiol Immunol & Pathol, Ft Collins, CO 80523 USA

来源：

VIROLOGY | 2011年 / 410卷 / 01期

关键词：

West Nile virus; Flavivirus; Pre-membrane protein; Human monoclonal antibodies; NONINFECTIOUS RECOMBINANT ANTIGEN; JAPANESE ENCEPHALITIS-VIRUS; CROSS-REACTIVE EPITOPES; DENGUE VIRUS; ENVELOPE PROTEIN; IMMUNE-RESPONSE; FLAVIVIRUS; INFECTION; MICE; CHALLENGE;

D O I：

10.1016/j.virol.2010.10.033

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Hybridoma cell lines (2E8, 8G8 and 5G12) producing fully human monoclonal antibodies (hMAbs) specific for the pre-membrane (prM) protein of West Nile virus (WNV) were prepared using a human fusion partner cell line, MFP-2, and human peripheral blood lymphocytes from a blood donor diagnosed with WNV fever in 2004. Using site-directed mutagenesis of a WNV-like particle (VLP) we identified 4 amino acid residues in the prM protein unique to WNV and important in the binding of these hMAbs to the VLP. Residues V19 and 133 are important epitopes for the binding of all three hMAbs. Mutations at residue, T20 and T24 affected the binding of hMAbs, 8G8 and 5G12 only. These hMAbs did not significantly protect AG129 interferon-deficient mice or Swiss Webster outbred mice from WNV infection. Published by Elsevier Inc.

引用

页码：30 / 37

页数：8

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