Facile Synthetic Route for Surface-Functionalized Magnetic Nanoparticles: Cell Labeling and Magnetic Resonance Imaging Studies

被引：73

作者：

Chung, Hyun Jung ^{[2
]}

Lee, Haeshin ^{[3
,4
]}

Bae, Ki Hyun ^{[2
]}

Lee, Yuhan ^{[2
]}

Park, Jongnam ^{[5
]}

Cho, Seung-Woo ^{[6
]}

Hwang, Jin Young ^{[7
]}

Park, Hyunwook ^{[7
]}

Langer, Robert ^{[1
,8
,9
]}

Anderson, Daniel ^{[1
,8
,9
]}

Park, Tae Gwan ^{[2
,4
]}

机构：

[1] MIT, David H Koch Inst Integrat Canc Res, Cambridge, MA 02139 USA

[2] Korea Adv Inst Sci & Technol, Dept Biol Sci, Taejon 305701, South Korea

[3] Korea Adv Inst Sci & Technol, Dept Chem, Taejon 305701, South Korea

[4] Korea Adv Inst Sci & Technol, Grad Sch Nanosci & Technol, Taejon 305701, South Korea

[5] Ulsan Natl Inst Sci & Technol, Ulsan 689805, South Korea

[6] Yonsei Univ, Dept Biotechnol, Seoul 120749, South Korea

[7] Korea Adv Inst Sci & Technol, Dept Elect Engn, Taejon 305701, South Korea

[8] MIT, Dept Chem Engn, Cambridge, MA 02139 USA

[9] MIT, Harvard Mit Div Hlth Sci & Technol, Cambridge, MA 02139 USA

来源：

ACS NANO | 2011年 / 5卷 / 06期

基金：

新加坡国家研究基金会;

关键词：

iron oxide nanoparticles; dispersion; PEG; surface functionalization; MRI; IRON-OXIDE NANOPARTICLES; PARTICLE-SIZE; NANOCRYSTALS; DELIVERY;

D O I：

10.1021/nn201198f

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Currently available methods to stably disperse iron oxide nanoparticles (IONPs) in aqueous solution need to be improved due to potential aggregation, reduction of superparamagnetism, and the use of toxic reagents. Herein, we present a facile strategy for aqueous transfer and dispersion of organic-synthesized IONPs using only polyethylene glycol (PEG), a biocompatible polymer. A library of PEG derivatives was screened, and it was determined that amine-functionalized six-armed PEG, 6(PEG-NH2), was the most effective dispersion agent. The 6(PEG-NH2)-modified IONPs (IONP-6PEG) were stable after extensive washing, exhibited high superparamagnetism, and could be used as a platform material for secondary surface functionalization with bioactive polymers. IONP-6PEG biofunctionalized with hyaluronic acid (IONP-6PEG-HA) was shown to specifically label mesenthymal stem cells and demonstrate MR contrast potential with high r(2) relaxivity (442.7 s(-1)mM(-1)) compared to the commercially available Feridex (182.1 s(-1)mM(-1)).

引用

页码：4329 / 4336

页数：8

共 30 条

[1] Bioinspired Synthesis and Characterization of Gadolinium-Labeled Magnetite Nanoparticles for Dual Contrast T1- and T2-Weighted Magnetic Resonance Imaging [J].

Bae, Ki Hyun ;

Kim, Young Beom ;

Lee, Yuhan ;

Hwang, JinYoung ;

Park, HyunWook ;

Park, Tae Gwan .

BIOCONJUGATE CHEMISTRY, 2010, 21 (03) :505-512

[2] Magnetodendrimers allow endosomal magnetic labeling and in vivo tracking of stem cells [J].

Bulte, JWM ;

Douglas, T ;

Witwer, B ;

Zhang, SC ;

Strable, E ;

Lewis, BK ;

Zywicke, H ;

Miller, B ;

van Gelderen, P ;

Moskowitz, BM ;

Duncan, ID ;

Frank, JA .

NATURE BIOTECHNOLOGY, 2001, 19 (12) :1141-1147

[3] Nanoplatforms for targeted molecular imaging in living subjects [J].

Cai, Weibo ;

Chen, Xiaoyuan .

SMALL, 2007, 3 (11) :1840-1854

[4] Reexamining the effects of particle size and surface chemistry on the magnetic properties of iron oxide nanocrystals: New insights into spin disorder and proton relaxivity [J].

Duan, Hongwei ;

Kuang, Min ;

Wang, Xiaoxia ;

Wang, Y. Andrew ;

Mao, Hui ;

Nie, Shuming .

JOURNAL OF PHYSICAL CHEMISTRY C, 2008, 112 (22) :8127-8131

[5] Synthesis and surface engineering of iron oxide nanoparticles for biomedical applications [J].

Gupta, AK ;

Gupta, M .

BIOMATERIALS, 2005, 26 (18) :3995-4021

[6] Super-stable, high-quality Fe3O4 dendron-nanocrystals dispersible in both organic and aqueous solutions [J].

Kim, M ;

Chen, YF ;

Liu, YC ;

Peng, XG .

ADVANCED MATERIALS, 2005, 17 (11) :1429-+

[7]

Kircher MF, 2003, CANCER RES, V63, P8122

[8] In vivo magnetic resonance imaging of mesenchymal stem cells in myocardial infarction [J].

Kraitchman, DL ;

Heldman, AW ;

Atalar, E ;

Amado, LC ;

Martin, BJ ;

Pittenger, MF ;

Hare, JM ;

Bulte, JWM .

CIRCULATION, 2003, 107 (18) :2290-2293

[9] Coating thickness of magnetic iron oxide nanoparticles affects R2 relaxivity [J].

LaConte, Leslie E. W. ;

Nitin, Nitin ;

Zurkiya, Omar ;

Caruntu, Daniela ;

O'Connor, Charles J. ;

Hu, Xiaoping ;

Bao, Gang .

JOURNAL OF MAGNETIC RESONANCE IMAGING, 2007, 26 (06) :1634-1641

[10] Magnetic iron oxide nanoparticles: Synthesis, stabilization, vectorization, physicochemical characterizations, and biological applications [J].

Laurent, Sophie ;

Forge, Delphine ;

Port, Marc ;

Roch, Alain ;

Robic, Caroline ;

Elst, Luce Vander ;

Muller, Robert N. .

CHEMICAL REVIEWS, 2008, 108 (06) :2064-2110

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