Antibacterial effect of silver nanoparticles on Staphylococcus aureus

被引：235

作者：

Mirzajani, Fateme ^{[1
]}

Ghassempour, Alireza ^{[1
]}

Aliahmadi, Atousa ^{[2
,3
]}

Esmaeili, Mohammad Ali ^{[2
]}

机构：

[1] Shahid Beheshti Univ, Med Plants & Drugs Res Inst, Dept Phytochem, Tehran, Iran

[2] Shahid Beheshti Univ, Med Plants & Drugs Res Inst, Dept Biol, Tehran, Iran

[3] Univ Isfahan, Fac Sci, Dept Biol, Esfahan, Iran

来源：

RESEARCH IN MICROBIOLOGY | 2011年 / 162卷 / 05期

关键词：

Antibactericidal mechanism; Muramic acid; Peptidoglycan; Silver nanoparticles; Staphylococcus aureus; ESCHERICHIA-COLI; MURAMIC ACID; ION; NANOSILVER; MECHANISM; TOXICITY;

D O I：

10.1016/j.resmic.2011.04.009

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Antibacterial activity of silver nanoparticles (AgNPs) was investigated using Staphylococcus aureus PTCC1431 as a model of Gram-positive bacteria. The mechanism of antibacterial activity of AgNPs was then studied by analyzing the growth, morphology, and molecular variations in the cell wall. Experimental data showed that AgNPs at a concentration of 4 mu g/ml completely inhibited bacterial growth. Transmission electron microscopy results confirmed cell wall damage produced by AgNPs as well as accumulation of AgNPs in the bacterial membrane. Meanwhile, the AgNP-treated bacteria were monitored by circular dichroism to reveal peptidoglycan variations. Some degree of variation in the alpha-helix position of the peptide chain was observed. Moreover, increasing the AgNP concentration to 8 mu g/ml resulted in release of muramic acid (MA) into the medium, which could be attributed to cell wall distraction. A gas chromatography-tandem mass spectrometry analysis and release of MA, as a bacterial indicator, showed that glycan strands may also be decomposed as a result of AgNP treatment. (C) 2011 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.

引用

页码：542 / 549

页数：8

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