Correlations among measles virus-specific antibody, lymphoproliferation and Th1/Th2 cytokine responses following measles-mumps-rubella-II (MMR-II) vaccination

被引:33
作者
Dhiman, N
Ovsyannikova, IG
Ryan, JE
Jacobson, RM
Vierkant, RA
Pankratz, VS
Jacobsen, SJ
Poland, GA
机构
[1] Mayo Clin, Coll Med, Mayo Clin & Mayo Fdn, Mayo Vaccine Res GrpProgram Translat Immunovirol, Rochester, MN 55905 USA
[2] Mayo Clin, Coll Med, Dept Pediat & Adolescent Med, Rochester, MN 55905 USA
[3] Mayo Clin, Coll Med, Dept Hlth Sci, Rochester, MN 55905 USA
关键词
antibody; cellular immunity; cytokines; lymphoproliferation; measles;
D O I
10.1111/j.1365-2249.2005.02931.x
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Immunity to measles is conferred by the interplay of humoral and cellular immune responses, the latter being critical in maintaining long-term recall response. Therefore, it is important to evaluate measles-specific humoral and cellular immunity in populations several years after vaccination and understand the correlations among these measures of immunity. We examined measles-specific antibodies, lymphoproliferation and the Th1/Th2 signature cytokines, interferon (IFN)-gamma and interleukin (IL)-4, in a population-based cohort of healthy children from Olmsted County, Minnesota after two doses of measles-mumps-rubella-II (MMR-II) vaccine. We detected positive measures of measles-specific cellular and humoral immunity in the majority of our study population. However, a small proportion of subjects demonstrated an immune response skewed towards the Th2 type, characterized by the presence of either IL-4 and/or measles-specific antibodies and a lack of IFN-gamma production. Further, we observed a significant positive correlation between lymphoproliferation and secretion of IFN-gamma (r = 0(.)20, P = 0(.)0002) and IL-4 (r = 0(.)15, P = 0(.)005). Measles antibody levels were correlated with lymphoproliferation (r = 0(.)12, P = 0(.)03), but lacked correlation to either cytokine type. In conclusion, we demonstrated the presence of both long-term cellular and humoral responses after MMR-II vaccination in a significant proportion of study subjects. Further, a positive correlation between lymphoproliferation and IL-4 and IFN-gamma suggests that immunity to measles may be maintained by both Th1 and Th2 cells. We speculate that the Th2 biased response observed in a subset of our subjects may be insufficient to provide long-term immunity against measles. Further examination of the determinants of Th1 versus Th2 skewing of the immune response and long-term follow-up is needed.
引用
收藏
页码:498 / 504
页数:7
相关论文
共 47 条
[21]  
JACOBSON RM, 2004, ACTA PAEDIATR, V445, P1
[22]   SEVERE MEASLES IN IMMUNOCOMPROMISED PATIENTS [J].
KAPLAN, LJ ;
DAUM, RS ;
SMARON, M ;
MCCARTHY, CA .
JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, 1992, 267 (09) :1237-1241
[23]   Impact of measles virus dendritic-cell infection on Th-cell polarization in vitro [J].
Klagge, IM ;
Abt, M ;
Fries, B ;
Schneider-Schaulies, S .
JOURNAL OF GENERAL VIROLOGY, 2004, 85 :3239-3247
[24]  
Markowitz LE, 1994, VACCINES, P229
[25]  
MARKOWITZ LE, 1998, J INFECT DIS, V158, P480
[26]   Herpes simplex virus vaccines - Why don't antibodies protect? [J].
Mascola, JR .
JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, 1999, 282 (04) :379-380
[27]   ATTENUATED MEASLES VACCINE IN CHILDREN WITH ACUTE LEUKEMIA [J].
MITUS, A ;
HOLLOWAY, A ;
ENDERS, JF ;
EVANS, AE .
AMERICAN JOURNAL OF DISEASES OF CHILDREN, 1962, 103 (03) :413-&
[28]   Decrease in measles virus-specific CD4 T cell memory in vaccinated subjects [J].
Naniche, D ;
Garenne, M ;
Rae, C ;
Manchester, M ;
Buchta, R ;
Brodine, SK ;
Oldstone, MBA .
JOURNAL OF INFECTIOUS DISEASES, 2004, 190 (08) :1387-1395
[29]   APPEARANCE AND PERSISTENCE OF ANTIBODIES AGAINST DIFFERENT VIRUS COMPONENTS AFTER REGULAR MEASLES INFECTIONS [J].
NORRBY, E ;
GOLLMAR, Y .
INFECTION AND IMMUNITY, 1972, 6 (03) :240-&
[30]   Human leukocyte antigen class II alleles and rubella-specific humoral and cell-mediated immunity following measles-mumps-rubella-II vaccination [J].
Ovsyannikova, IG ;
Jacobson, RM ;
Vierkant, RA ;
Jacobsen, SJ ;
Pankratz, VS ;
Poland, GA .
JOURNAL OF INFECTIOUS DISEASES, 2005, 191 (04) :515-519