Chemical force microscopy with active enzymes

被引：27

作者：

Fiorini, M ^{[1
]}

McKendry, R ^{[1
]}

Cooper, MA ^{[1
]}

Rayment, T ^{[1
]}

Abell, C ^{[1
]}

机构：

[1] Univ Cambridge, Dept Chem, Cambridge CB2 1EW, England

来源：

BIOPHYSICAL JOURNAL | 2001年 / 80卷 / 05期

关键词：

D O I：

10.1016/S0006-3495(01)76215-7

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

The adhesion forces have been measured between an atomic force microscope tip derivatized with an active enzyme, shikimate kinase, and an ATP mimic immobilized on a gold surface. Experiments with competitive binding of other ligands in solution show that the observed adhesion forces arise predominantly from specific interactions between the immobilized enzyme and surface-bound adenine derivative. These experiments represent a step in the development of a screening methodology based upon chemical force microscopy.

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收藏

页码：2471 / 2476

页数：6

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