Continuous culture - making a comeback?

被引:195
作者
Hoskisson, PA
Hobbs, G
机构
[1] Liverpool John Moores Univ, Sch Biomol Sci, Liverpool L3 3AF, Merseyside, England
[2] Univ Aberdeen, Inst Med Sci, Dept Mol & Cell Biol, Aberdeen AB25 2ZD, Scotland
[3] Liverpool John Moores Univ, Sch Biomol Sci, Liverpool L3 3AF, Merseyside, England
来源
MICROBIOLOGY-SGM | 2005年 / 151卷
关键词
D O I
10.1099/mic.0.27924-0
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The heyday of continuous culture was in the 1960s, when its versatility and reproducibility were used to address fundamental problems in diverse microbiological fields such as biochemistry, ecology, genetics and physiology. The advent of molecular genetics in the 1970s and 1980s led to a decline in the popularity of continuous culture as a standard laboratory tool. The current trend of studying global proteomics, transcriptomics and metabolomics requires reproducible, reliable and biologically homogeneous datasets with which to approach a given problem. The use of continuous culture techniques can aid the acquisition of such data, and continuous cultures offer advantages over biologically heterogeneous batch cultures, where secondary growth and stress effects can often mask subtle physiological differences and trends. This review is intended to remind microbiologists of the value of continuous cultivation in a wide range of biological investigations, and describes some advantages and recent advances in applications of continuous culture in post-genomic studies.
引用
收藏
页码:3153 / 3159
页数:7
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