Type D retrovirus gag polyprotein interacts with the cytosolic chaperonin TRiC

被引:52
作者
Hong, S
Choi, G
Park, S
Chung, AS
Hunter, E
Rhee, SS
机构
[1] Samsung Biomed Res Inst, Mol Virol Lab, Seoul, South Korea
[2] Korea Adv Inst Sci & Technol, Dept Sci Biol, Taejon 305701, South Korea
[3] Univ Alabama Birmingham, Dept Microbiol, Birmingham, AL 35294 USA
关键词
D O I
10.1128/JVI.75.6.2526-2534.2001
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The carboxy terminus-encoding portion of the gag gene of Mason-Pfizer monkey virus (M-PMV), the prototype immunosuppressive primate type D retrovirus, encodes a 36-amino-acid, proline-rich protein domain that, in the mature virion, becomes the p4 capsid protein. The p4 domain has no known role in M-PMV replication. We found that two mutants,vith premature termination codons that remove half or all of the p4 domain produced lower levels of stable Gag protein and of self-assembled capsids. Interestingly, yeast two-hybrid screening revealed that p4 specifically interacted with TCP-1 gamma, a subunit of the chaperonin TRiC (TCP-I ring complex). TRiC is a cytosolic chaperonin that is known to be involved in both folding and subunit assembly of a variety of cellular proteins. TCP-1 gamma also associated with high specificity with the M-PMV pp24/16-p12 domain and human immunodeficiency virus p6. Moreover, in cells, Gag polyprotein associated with the TRiC chaperonin complex and this association depended on ATP hydrolysis. In the p4 truncation mutants, the Gag-TRiC association was significantly reduced. These results strongly suggest that cytosolic chaperonin TRiC is involved in Gag folding and/or capsid assembly. We propose that TRiC associates transiently with nascent M-PMV Gag molecules to assist in their folding. Consequently, properly folded Gag molecules carry out the intermolecular interactions involved in self-assembly of the immature capsid.
引用
收藏
页码:2526 / 2534
页数:9
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