Effect of ethanol/trisodium citrate lock on microorganisms causing hemodialysis catheter-related infections

被引:13
作者
Takla, T. A. [1 ]
Zelenitsky, S. A. [1 ,2 ]
Vercaigne, L. M. [1 ,3 ]
机构
[1] Univ Manitoba, Fac Pharm, Winnipeg, MB R3T 2N2, Canada
[2] St Boniface Gen Hosp, Winnipeg, MB R2H 2A6, Canada
[3] Manitoba Renal Program, Winnipeg, MB, Canada
关键词
hemodialysis; vascular access; infection; catheters;
D O I
10.1177/112972980700800408
中图分类号
R6 [外科学];
学科分类号
1002 ; 100210 ;
摘要
Purpose: This in vitro study tested the effectiveness of a novel 30% ethanol/4% trisodium citrate (TSC) lock solution against the most common pathogens causing hemodialysis catheter-related infections. Methods: Clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA) (n=4), methicillin-sensitive S. aureus (MSSA) (n=8), methicillin-resistant Staphylococcus epidermidis (MORSE) (n=8), Pseudomonas aeruginosa (n=4) and Escherichia coli (n=4) were tested in duplicate. Bacterial suspensions of each isolate were made in a control solution of normal saline and Mueller-Hinton broth (MHB), and in a lock solution of ethanol 30%, TSC 4% and MHB. Suspensions were incubated at 37 degrees C for 48 h. Colony counts were determined from samples collected at t=0 h (before exposure to the ethanol/TSC lock), t=1 h (one hour after exposure to the ethanol/TSC lock), t=24 h and t=48 h. To confirm the absence of viable organisms in the lock solution, the remaining volume at 48 h was filtered through a 0.45 pin filter. The filter was rinsed with 15 mL sterile water and plated on tryptic soy agar (TSA). Results: All controls demonstrated significant growth over 48 h. In the lock solutions, initial inocula were reduced to 0 viable colonies by t=1 h (6-log kill), and there was no growth at t=24 and 48 h. Filtering of lock solutions also showed no growth. These results were consistent among duplicates of all isolates. Conclusions: The 30% ethanol/4% TSC lock solution consistently eradicated MRSA, MSSA, MORSE, R aeruginosa and E. coli within 1 h of exposure. Experiments are currently underway to test this novel lock solution on preventing biofilm production by these pathogens.
引用
收藏
页码:262 / 267
页数:6
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