Immunohistochemical classification of skeletal muscle fibers

被引：32

作者：

Hori, A

Ishihara, A ^{[1
]}

Kobayashi, S

Ibata, Y

机构：

[1] Kyoto Univ, Grad Sch Informat, Dept Bioinformat, Kyoto 6068501, Japan

[2] Kyoto Univ, Fac Integrated Human Studies, Neurochem Lab, Kyoto 6068501, Japan

[3] Kyoto Prefectural Univ Med, Dept Anat & Neurobiol, Kyoto 6020841, Japan

来源：

ACTA HISTOCHEMICA ET CYTOCHEMICA | 1998年 / 31卷 / 05期

关键词：

enzyme histochemistry; immunohistochemistry; muscle fiber type; myosin heavy; chain isoform; skeletal muscle;

D O I：

10.1267/ahc.31.375

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Mammalian skeletal muscle fibers have several types of myosin heavy chain (MHC) isoforms. Immunohistochemical techniques enable us to identify the MHC isoform type in a single muscle fiber, i.e., MHCI, MHCIIa, MHCIIx, or MHCIIb. In addition, hybrid single fibers having more than one MHC isoform type can be classified using immunohistochemistry. The presence of different MHC isoform(s) in a single fiber correlates with the maximal contractile velocity and the enzyme histochemical reaction of the myofibrillar adenosine triphosphatase. The adaptive response of the skeletal muscle is the basis of the fiber-type transformation depending on the phenotypic expression pattern in several MHC isoforms. In this review article, we address the availability of the monoclonal antibodies which recognize specific MHC isoform(s) in a single fiber.

引用

页码：375 / 384

页数：10

共 96 条

[41] ALL MEMBERS OF THE MHC MULTIGENE FAMILY RESPOND TO THYROID-HORMONE IN A HIGHLY TISSUE-SPECIFIC MANNER [J].