Regulation of hepcidin: Insights from biochemical analyses on human serum samples

被引：96

作者：

Kemna, Erwin H. J. M. ^{[1
]}

Kartikasari, April E. R. ^{[1
]}

van Tits, Lambertus J. H. ^{[2
]}

Pickkers, Peter ^{[3
]}

Tjalsma, Harold ^{[1
]}

Swinkels, Dorine W. ^{[1
]}

机构：

[1] Radboud Univ Nijmegen, Med Ctr, Dept Clin Chem 441, NL-6500 HB Nijmegen, Netherlands

[2] Radboud Univ Nijmegen, Med Ctr, Dept Internal Med, NL-6525 ED Nijmegen, Netherlands

[3] Radboud Univ Nijmegen, Med Ctr, Dept Intens Care Med, NL-6525 ED Nijmegen, Netherlands

来源：

BLOOD CELLS MOLECULES AND DISEASES | 2008年 / 40卷 / 03期

关键词：

hepcidin; iron deficiency; sTfR; thalassemia; iron;

D O I：

10.1016/j.bcmd.2007.10.002

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Knowledge of hepcidin regulation is foremost gained by in vitro studies. We aimed to translate this knowledge into the human in vivo situation. Therefore, we measured serum markers as transferrin saturation (TS), soluble transferrin receptor (sTfR), and C-reactive protein (CRP) in parallel with hepcidin and prohepcidin in patients with iron metabolism disorders and controls. To assess sTfR as erythropoietic activity-associated factor in hepcidin regulation, we studied its influence on hepcidin expression in HepG2 cells. Results showed that sTfR highly associates with erythropoietic activity that strongly interfered with the iron store regulation of hepcidin. HepG2 expression results display an inverse association between hepcidin and sTfR. Inflammation was strongly related to increased hepcidin levels regardless of the iron store and erythropoietic activity status. In contrast, prohepcidin failed to correlate to any other parameter. In conclusion, these studies verify that previous conclusions based on in vitro studies on hepicidin regulation are also likely to apply to human patients. This is underscored by a simple algorithm, based on parameters reflecting the main regulating pathways, that accurately predict the actual measured hepcidin levels. Future studies are needed to validate the combined utility of this predictive algorithm together with actual measured hepcidin levels in clinical diagnosis. (C) 2007 Elsevier Inc. All rights reserved.

引用

页码：339 / 346

页数：8

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