Evidence for a tetrameric form of iceberg lettuce (Lactuca sativa L.) polyphenol oxidase:: Purification and characterization

被引：37

作者：

Chazarra, S ^{[1
]}

García-Carmona, F ^{[1
]}

Cabanes, J ^{[1
]}

机构：

[1] Univ Murcia, Dept Bioquim & Biol Mol, Unidad Docente Biol, E-30071 Murcia, Spain

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2001年 / 49卷 / 10期

关键词：

polyphenol oxidase; lettuce; purification;

D O I：

10.1021/jf0100301

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Polyphenol oxidase from iceberg lettuce (Lactuca sativa L.) chloroplasts was released from the thylakoid-membrane by sonication, and it was extensively purified to homogeneity as judged by SDS-PAGE. Purification was achieved by ammonium sulfate fractionation, gel-filtration chromatography, and ion-exchange chromatography. Two molecular forms were separated by gel-filtration chromatography with apparent molecular masses of 188 and 49 kDa. Both forms were characterized by sedimentation analysis with S-20,S-W values of 10.2 and 4.1 S, respectively. For the high-molecular weight form purified to homogeneity, denaturing SDS-PAGE indicated a molecular mass of 60 kDa. Thus, from these data we suggest that lettuce polyphenol oxidase is a tetramer of identical subunits.

引用

页码：4870 / 4875

页数：6

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