Fluorescence spectroscopy of single molecules under ambient conditions:: Methodology and technology

被引：76

作者：

Böhmer, M ^{[1
]}

Enderlein, J ^{[1
]}

机构：

[1] Forschungszentrum Julich, D-52425 Julich, Germany

来源：

CHEMPHYSCHEM | 2003年 / 4卷 / 08期

关键词：

fluorescence spectroscopy; FRET (fluorescence resonance energy transfer); single-molecule studies;

D O I：

10.1002/cphc.200200565

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

This review presents an overview of the fluorescence detection and spectroscopy of single molecules (SMS) in the liquids and on surfaces under ambient conditions. The various techniques of SMS, such as confocal epifluorescene detection and wide-field imaging are presented and discussed together with the different methods of data analysis such as fluorescence correlation spectroscopy and burst-by-burst analysis. Selected applications of the various techniques in physics, chemistry and biology are described.

引用

页码：793 / 808

页数：16

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