Interactions of exercise training and lipoic acid on skeletal muscle glucose transport in obese Zucker rats

被引:38
作者
Saengsirisuwan, V [1 ]
Kinnick, TR [1 ]
Schmit, MB [1 ]
Henriksen, EJ [1 ]
机构
[1] Univ Arizona, Coll Med, Dept Physiol, Muscle Metab Lab, Tucson, AZ 85721 USA
关键词
insulin resistance; oxidative stress; glucose tolerance; GLUT-4; protein; protein carbonyls;
D O I
10.1152/jappl.2001.91.1.145
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
Exercise training (ET) or the antioxidant R(+)-alpha -lipoic acid (R-ALA) individually increases insulin action in the insulin-resistant obese Zucker rat. The purpose of the present study was to determine the interactions of ET and R-ALA on insulin action and oxidative stress in skeletal muscle of the obese Zucker rat. Animals either remained sedentary, received R-ALA (30 mg.kg body wt(-1).day(-1)), performed ET (treadmill running), or underwent both R-ALA treatment and ET for 6 wk. During an oral glucose tolerance test, ET alone or in combination with R-ALA resulted in a significant lowering of the glucose (26-32%) and insulin (29-30%) responses compared with sedentary controls. R-ALA alone decreased (19%) the glucose-insulin index (indicative of increased insulin sensitivity), and this parameter was reduced (48-52%) to the greatest extent in the ET and combined treatment groups. ET or R-ALA individually increased insulin-mediated glucose transport activity in isolated epitrochlearis (44-48%) and soleus (37-57%) muscles. The greatest increases in insulin action in these muscles (80 and 99%, respectively) were observed in the combined treatment group. Whereas the improvement in insulin-mediated glucose transport in soleus due to R-ALA was associated with decreased protein carbonyl levels (an index of oxidative stress), improvement because of ET was associated with decreased protein carbonyls as well as enhanced GLUT-4 protein. However, there was no interactive effect of ET and R-ALA on GLUT-4 protein or protein carbonyl levels. These results indicate that ET and R-ALA interact in an additive fashion to improve insulin action in insulin-resistant skeletal muscle. Because the further improvement in muscle glucose transport in the combined group was not associated with additional upregulation of GLUT-4 protein or a further reduction in oxidative stress, the mechanism for this interaction must be due to additional, as yet unidentified, factors.
引用
收藏
页码:145 / 153
页数:9
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