Efficiency of DNA barcodes for species delimitation: A case in Pterygiella Oliv. (Orobanchaceae)

被引：28

作者：

Dong, Li-Na ^{[1
,2
,3
]}

Wortley, Alexandra H. ^{[4
]}

Wang, Hong ^{[1
,2
]}

Li, De-Zhu ^{[1
,2
]}

Lu, Lu ^{[1
,2
]}

机构：

[1] Chinese Acad Sci, Key Lab Biodivers & Biogeog, Germplasm Bank Wild Species, Kunming Inst Bot, Kunming 650204, Yunnan, Peoples R China

[2] Chinese Acad Sci, Plant Germplasm & Genom Ctr, Germplasm Bank Wild Species, Kunming Inst Bot, Kunming 650204, Yunnan, Peoples R China

[3] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

[4] Royal Bot Garden Edinburgh, Edinburgh EH3 5LR, Midlothian, Scotland

来源：

JOURNAL OF SYSTEMATICS AND EVOLUTION | 2011年 / 49卷 / 03期

基金：

中国国家自然科学基金;

关键词：

compensatory base changes (CBCs); DNA barcode; ITS-2; non-monophyletic species; Pterygiella Oliv; secondary structure; NUCLEAR RIBOSOMAL DNA; WEB SERVER; ITS2; SEQUENCE; IDENTIFICATION; EVOLUTIONARY; PLANTS; NRDNA; PREDICTION; PHYLOGENY;

D O I：

10.1111/j.1759-6831.2011.00124.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 [植物学];

摘要：

DNA barcoding is becoming an increasingly popular means to identify species. The obscure discrimination in the genus Pterygiella calls into question the re-assessment of the criterion for species delimitation. We collected 20 individuals, representing all five described species of this genus in its distributional range. The aim was to use three proposed barcode DNA regions (rbcL, matK, and ITS) to diagnose Pterygiella species, and examine which barcode is more suitable for discerning the congeneric and related species. The results showed that the core barcodes matK and rbcL were comparatively less effective. However, the ITS region, especially ITS-1 and ITS-2, successfully identified all species in the genus. Furthermore, the secondary structure of ITS-2 RNA, especially compensatory base changes, appears complementary to classical primary sequence analysis for DNA barcoding.

引用

页码：189 / 202

页数：14

共 69 条

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