Functional interaction between two transcription factors involved in the developmental regulation of a small heat stress protein gene promoter

被引：73

作者：

Díaz-Martín, J ^{[1
]}

Almoguera, CN ^{[1
]}

Prieto-Dapena, P ^{[1
]}

Espinosa, JM ^{[1
]}

Jordano, J ^{[1
]}

机构：

[1] Consejo Super Invest Cientificas, Inst Recursos Nat & Agrobiol, Seville 41080, Spain

来源：

PLANT PHYSIOLOGY | 2005年 / 139卷 / 03期

关键词：

D O I：

10.1104/pp.105.069963

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Hahsp17.6G1 is the promoter of a small heat stress protein (sHSP) from sunflower (Helianthus annuus) that is activated during zygotic embryogenesis, but which does not respond to heat stress. We report here the cloning of a transcription factor (TF), sunflower drought-responsive element binding factor 2 (HaDREB2), by one-hybrid interaction with functional cis-elements in Hahsp17.6G1. We have analyzed the functional interaction between HaDREB2 and a second transcription factor, sunflower heat stress factor A9 (HaHSFA9), which was previously assigned to the regulation of Hahsp17.6G1. HaDREB2 and HaHSFA9 synergistically trans-activate the Hahsp17.6G1 promoter in bombarded sunflower embryos. This synergistic interaction is heat stress factor (HSF) specific and requires the binding of both factors to the promoter. The C-terminal region of HaHSFA9 is sufficient for the HSF specificity. Our results represent an example of a functional interaction between members of the Apetala 2 (HaDREB2) and HSF (HaHSFA9) families of transcription factors. We suggest new roles in zygotic embryogenesis for specific members of the AP2 transcription factor family.

引用

页码：1483 / 1494

页数：12

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