Transcript copy number estimation using a mouse whole-genome oligonucleotide microarray

被引：93

作者：

Carter, MG

Sharov, AA

VanBuren, V

Dudekula, DB

Carmack, CE

Nelson, C

Ko, MSH

机构：

[1] NIA, Dev Genom & Aging Sect, Genet Lab, Baltimore, MD 21224 USA

[2] Agilent Technol, Palo Alto, CA 94304 USA

来源：

GENOME BIOLOGY | 2005年 / 6卷 / 07期

关键词：

D O I：

10.1186/gb-2005-6-7-r61

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The ability to quantitatively measure the expression of all genes in a given tissue or cell with a single assay is an exciting promise of gene-expression profiling technology. An in situ-synthesized 60-mer oligonucleotide microarray designed to detect transcripts from all mouse genes was validated, as well as a set of exogenous RNA controls derived from the yeast genome ( made freely available without restriction), which allow quantitative estimation of absolute endogenous transcript abundance.

引用

页数：12

共 28 条

[1] Gene Ontology: tool for the unification of biology
Ashburner, M
Ball, CA
Blake, JA
Botstein, D
Butler, H
Cherry, JM
Davis, AP
Dolinski, K
Dwight, SS
Eppig, JT
Harris, MA
Hill, DP
Issel-Tarver, L
Kasarskis, A
Lewis, S
Matese, JC
Richardson, JE
Ringwald, M
Rubin, GM
Sherlock, G
[J]. NATURE GENETICS, 2000, 25 (01) : 25 - 29
[2] ANALYSIS OF COMPLEXITY AND DIVERSITY OF MESSENGER-RNA FROM CHICKEN LIVER AND OVIDUCT
AXEL, R
FEIGELSON, P
SCHUTZ, G
[J]. CELL, 1976, 7 (02) : 247 - 254
[3] 3 ABUNDANCE CLASSES IN HELA-CELL MESSENGER-RNA
BISHOP, JO
MORTON, JG
ROSBASH, M
RICHARDSON, M
[J]. NATURE, 1974, 250 (5463) : 199 - 204
[4] Expression profiling of the schizont and trophozoite stages of Plasmodium falciparum with a long-oligonucleotide microarray -: art. no. R9
Bozdech, Z
Zhu, JC
Joachimiak, MP
Cohen, FE
Pulliam, B
DeRisi, JL
[J]. GENOME BIOLOGY, 2003, 4 (02)
[5] ArrayExpress - a public repository for microarray gene expression data at the EBI
Brazma, A
Parkinson, H
Sarkans, U
Shojatalab, M
Vilo, J
Abeygunawardena, N
Holloway, E
Kapushesky, M
Kemmeren, P
Lara, GG
Oezcimen, A
Rocca-Serra, P
Sansone, SA
[J]. NUCLEIC ACIDS RESEARCH, 2003, 31 (01) : 68 - 71
[6] The NIA cDNA Project in mouse stem cells and early embryos
Carter, MG
Piao, YL
Dudekula, DB
Qian, Y
VanBuren, V
Sharov, AA
Tanaka, TS
Martin, PR
Bassey, UC
Stagg, CA
Aiba, K
Hamatani, T
Matoba, R
Kargul, GJ
Ko, MSH
[J]. COMPTES RENDUS BIOLOGIES, 2003, 326 (10-11) : 931 - 940
[7] In situ-synthesized novel microarray optimized for mouse stem cell and early developmental expression profiling
Carter, MG
Hamatani, T
Sharov, AA
Carmack, CE
Qian, Y
Aiba, K
Ko, NT
Dudekula, DB
Brzoska, PM
Hwang, SS
Ko, MSH
[J]. GENOME RESEARCH, 2003, 13 (05) : 1011 - 1021
[8] Measuring absolute expression with microarrays with a calibrated reference sample and an extended signal intensity range
Dudley, AM
Aach, J
Steffen, MA
Church, GM
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (11) : 7554 - 7559
[9] Gene Expression Omnibus: NCBI gene expression and hybridization array data repository
Edgar, R
Domrachev, M
Lash, AE
[J]. NUCLEIC ACIDS RESEARCH, 2002, 30 (01) : 207 - 210
[10] ESTGenes: Alternative splicing from ESTs in Ensembl
Eyras, E
Caccamo, M
Curwen, V
Clamp, M
[J]. GENOME RESEARCH, 2004, 14 (05) : 976 - 987

← 1 2 3 →