The graft response to transplantation: a gene expression profile analysis

被引：9

作者：

Christopher, K

Mueller, TF

DeFina, R

Liang, YR

Zhang, JH

Gentleman, R

Perkins, DL

机构：

[1] Harvard Univ, Brigham & Womens Hosp, Sch Med,Dept Med, Lab Mol Immunol, Boston, MA 02115 USA

[2] Harvard Univ, Sch Med, Dana Farber Canc Inst, Dept Biostat Sci,Biostat Unit, Boston, MA 02115 USA

来源：

PHYSIOLOGICAL GENOMICS | 2003年 / 15卷 / 01期

关键词：

gene regulation; rodent;

D O I：

10.1152/physiolgenomics.00139.2002

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Little is known regarding the graft response to transplantation injury. This study investigates the posttransplantation response of genes that are constitutively expressed in the heart. Constitutive heart and lymph node tissue-restricted gene expression was first analyzed with DNA microarrays. To demonstrate changes following transplantation in genes constitutively expressed in the heart, we performed vascularized murine heart transplants in allogeneic (BALB/c to B6), syngeneic ( B6 to B6), and alymphoid (BALB/c-RAG2(-/-) to B6-RAG1(-/-)) experimental groups. Temporal induction of genes posttransplant relative to constitutive expression was evaluated with DNA microarrays. Dendrograms and self-organizing maps were generated to determine the dissimilarity between the experimental groups and to identify subsets of differentially expressed genes within the groups, respectively. Expression patterns of selected genes were confirmed by real-time PCR. Biological processes were assigned to genes induced posttransplant using the Ann-Builder package via the Gene Ontology Database. Posttransplant, a shift was noted in genes classified as defense, communication, and metabolism. Our results identify novel components of the graft response to transplantation injury and rejection.

引用

页码：52 / 64

页数：13

共 27 条

[1] Gene Ontology: tool for the unification of biology [J].

Ashburner, M ;

Ball, CA ;

Blake, JA ;

Botstein, D ;

Butler, H ;

Cherry, JM ;

Davis, AP ;

Dolinski, K ;

Dwight, SS ;

Eppig, JT ;

Harris, MA ;

Hill, DP ;

Issel-Tarver, L ;

Kasarskis, A ;

Lewis, S ;

Matese, JC ;

Richardson, JE ;

Ringwald, M ;

Rubin, GM ;

Sherlock, G .

NATURE GENETICS, 2000, 25 (01) :25-29

[2] Eta-1 (osteopontin): An early component of type-1 (cell-mediated) immunity [J].

Ashkar, S ;

Weber, GF ;

Panoutsakopoulou, V ;

Sanchirico, ME ;

Jansson, M ;

Zawaideh, S ;

Rittling, SR ;

Denhardt, DT ;

Glimcher, MJ ;

Cantor, H .

SCIENCE, 2000, 287 (5454) :860-864

[3]

CHARLES CH, 1992, ONCOGENE, V7, P187

[4] Analysis of the innate and adaptive phases of allograft rejection by cluster analysis of transcriptional profiles [J].

Christopher, K ;

Mueller, TF ;

Ma, CY ;

Liang, YR ;

Perkins, DL .

JOURNAL OF IMMUNOLOGY, 2002, 169 (01) :522-530

[5]

CORRY RJ, 1973, TRANSPLANT P, V5, P733

[6]

de Bont N, 1999, J LIPID RES, V40, P680

[7] Gene program for cardiac cell survival induced by transient ischemia in conscious pigs [J].

Depre, C ;

Tomlinson, JE ;

Kudej, RK ;

Gaussin, V ;

Thompson, E ;

Kim, SJ ;

Vatner, DE ;

Topper, JN ;

Vatner, SF .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (16) :9336-9341

[8] Cluster analysis and display of genome-wide expression patterns [J].

Eisen, MB ;

Spellman, PT ;

Brown, PO ;

Botstein, D .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (25) :14863-14868

[9] Synthetic peptides which inhibit the interaction between C1q and immunoglobulin and prolong xenograft survival [J].

Fryer, JP ;

Leventhal, JR ;

Pao, W ;

Stadler, C ;

Jones, M ;

Walsh, T ;

Zhong, R ;

Zhang, Z ;

Wang, H ;

Goodman, DJ ;

Kurek, M ;

d'Apice, AJF ;

Blondin, B ;

Ivancic, D ;

Buckingham, F ;

Kaufman, D ;

Abecassis, M ;

Stuart, F ;

Anderson, BE .

TRANSPLANTATION, 2000, 70 (05) :828-836

[10] Suppression of fibrous adhesion by proteoglycan decorin [J].

Fukui, N ;

Fukuda, A ;

Kojima, K ;

Nakajima, K ;

Oda, H ;

Nakamura, K .

JOURNAL OF ORTHOPAEDIC RESEARCH, 2001, 19 (03) :456-462

← 1 2 3 →