PCR-based detection of an emerging avian pneumovirus in US turkey flocks

被引:17
作者
Dar, AM [1 ]
Tune, K
Munir, S
Panigrahy, B
Goyal, SM
Kapur, V
机构
[1] Univ Minnesota, Dept Vet Pathobiol, St Paul, MN 55108 USA
[2] Univ Minnesota, Dept Vet Diagnost Med, St Paul, MN 55108 USA
[3] Natl Vet Serv Lab, Ames, IA 50010 USA
关键词
D O I
10.1177/104063870101300303
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
Avian pneumovirus (APV) or turkey rhinotracheitis virus (TRTV) is an important respiratory pathogen of domesticated poultry in many countries in Europe, Africa, and Asia. Until recently, the United States was considered free of APV. In late 1996, an atypical upper respiratory tract infection appeared in turkey flocks in Colorado and shortly thereafter in turkey flocks in Minnesota. An avian pneumovirus (APV-US) that was serologically distinct from the previously described TRTV was isolated as the primary cause of the new syndrome. The nucleotide sequence of a fragment of the APV-US fusion gene was determined and used to develop a polymerase. chain reaction-based assay that specifically detects APV-US viral nucleic acid sequences in RNA extracts of tracheal swabs and turbinate homogenates. The assay is highly sensitive in that it can detect <0.01 TCID50 of APV. The availability of this assay enables the rapid and accurate determination of APV-US in infected poultry flocks.
引用
收藏
页码:201 / 205
页数:5
相关论文
共 25 条
  • [1] A reverse transcription-polymerase chain reaction assay for the detection of avian pneumovirus (Colorado strain)
    Ali, A
    Reynolds, DL
    [J]. AVIAN DISEASES, 1999, 43 (03) : 600 - 603
  • [2] A COMPARISON OF 3 METHODS FOR DETECTING ANTIBODIES TO TURKEY RHINOTRACHEITIS VIRUS
    BAXTERJONES, C
    GRANT, M
    JONES, RC
    WILDING, GP
    [J]. AVIAN PATHOLOGY, 1989, 18 (01) : 91 - 98
  • [3] Comparison of F-, G- and N-based RT-PCR protocols with conventional virological procedures for the detection and typing of turkey rhinotracheitis virus
    Bäyon-Auboyer, MH
    Jestin, V
    Toquin, D
    Cherbonnel, M
    Eterradossi, N
    [J]. ARCHIVES OF VIROLOGY, 1999, 144 (06) : 1091 - 1109
  • [4] Cavanagh D, 1997, ACTA VET HUNG, V45, P281
  • [5] THE PATHOGENESIS OF TURKEY RHINOTRACHEITIS VIRUS IN TURKEY POULTS INOCULATED WITH THE VIRUS ALONE OR TOGETHER WITH 2 STRAINS OF BACTERIA
    COOK, JKA
    ELLIS, MM
    HUGGINS, MB
    [J]. AVIAN PATHOLOGY, 1991, 20 (01) : 155 - 166
  • [6] ANTIGENIC DIFFERENTIATION OF STRAINS OF TURKEY RHINOTRACHEITIS VIRUS USING MONOCLONAL-ANTIBODIES
    COOK, JKA
    JONES, BV
    ELLIS, MM
    JING, L
    CAVANAGH, D
    [J]. AVIAN PATHOLOGY, 1993, 22 (02) : 257 - 273
  • [7] COOK JKA, 1990, P W POULT DIS C, V39, P42
  • [8] Molecular characterization of Brazilian avian pneumovirus isolates: comparison between immunochemiluminescent Southern blot and nested PCR
    Dani, MAC
    Durigon, EL
    Arns, CW
    [J]. JOURNAL OF VIROLOGICAL METHODS, 1999, 79 (02) : 237 - 241
  • [9] ETERRADOSSI N, 1992, VET REC, V131, P563
  • [10] HIGGINS DG, 1989, COMPUT APPL BIOSCI, V5, P151