SSRs isolated from apple can identify polymorphism and genetic diversity in pear

被引:186
作者
Yamamoto, T
Kimura, T
Sawamura, Y
Kotobuki, K
Ban, Y
Hayashi, T
Matsuta, N
机构
[1] Natl Inst Fruit Tree Sci, Dept Breeding, Tsukuba, Ibaraki 3058605, Japan
[2] Natl Ctr Seeds & Seedlings, Tsukuba, Ibaraki 3058605, Japan
关键词
Malusxdomestica borkh; pedigree analysis; Pyrus spp; sequence similarity; simple sequence repeats;
D O I
10.1007/s001220000524
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Apple simple sequence repeats (SSRs) were intergenerically applied to the characterization of 36 pear accessions, including 19 Japanese pears (Pyrus pyrifolia), 7 Chinese pears (P. bretschneideri, P. ussuriensis), 5 European pears (P. communis), 3 wild relatives (P. calleryana), and 2 hybrids between P. pyrifolia and P. communis. All of the tested SSR primers derived from apple produced discrete amplified fragments in all pear accessions. Nucleotide repeats were detected in the amplified bands by both Southern blot and sequencing analysis, and nucleotide sequences of pear were compared with those of apple. The differences in fragment size among pear or between pear and apple were, in many cases, due to the differences in repeat number. Interestingly, the DNA sequence of flanking regions in apple was highly conserved in pear. Hybrids from P. pyrifolia x P. communis showed one fragment inherited from each parent in all scorable cases, which suggested that each primer pair amplified fragments originating from the same locus. A total of 79 alleles were detected from seven SSR loci in pear, and all pear varieties except for the mutants could be differentiated. In conclusion, SSRs isolated from apple are highly conserved in pear and could be utilized as DNA markers in the latter genus.
引用
收藏
页码:865 / 870
页数:6
相关论文
共 25 条
[1]  
Bell R. L., 1990, Acta Horticulturae, P655
[2]  
Bell RL, 1996, FRUIT BREEDING, P441
[3]   Inheritance and linkage of isozyme loci in pear (Pyrus communis L) [J].
Chevreau, E ;
Leuliette, S ;
Gallet, M .
THEORETICAL AND APPLIED GENETICS, 1997, 94 (3-4) :498-506
[4]   AC/GT and AG/CT microsatellite repeats in peach [Prunus persica (L) Batsch]:: isolation, characterisation and cross-species amplification in Prunus [J].
Cipriani, G ;
Lot, G ;
Huang, WG ;
Marrazzo, MT ;
Peterlunger, E ;
Testolin, R .
THEORETICAL AND APPLIED GENETICS, 1999, 99 (1-2) :65-72
[5]   Polymorphic DNA markers in black cherry (Prunus serotina) are identified using sequences from sweet cherry, peach, and sour cherry [J].
Downey, SL ;
Iezzoni, AF .
JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE, 2000, 125 (01) :76-80
[6]   Simple sequence repeats for the genetic analysis of apple [J].
Gianfranceschi, L ;
Seglias, N ;
Tarchini, R ;
Komjanc, M ;
Gessler, C .
THEORETICAL AND APPLIED GENETICS, 1998, 96 (08) :1069-1076
[7]   Microsatellites in Malus X domestica (apple): Abundance, polymorphism and cultivar identification [J].
Guilford, P ;
Prakash, S ;
Zhu, JM ;
Rikkerink, E ;
Gardiner, S ;
Bassett, H ;
Forster, R .
THEORETICAL AND APPLIED GENETICS, 1997, 94 (02) :249-254
[8]   ADIANTUM-CAPILLUS-VENERIS CHLOROPLAST DNA CLONE BANK - AS USEFUL HETEROLOGOUS PROBES IN THE SYSTEMATICS OF THE LEPTOSPORANGIATE FERNS [J].
HASEBE, M ;
IWATSUKI, K .
AMERICAN FERN JOURNAL, 1990, 80 (01) :20-25
[9]   Incongruence between RFLPs of chloroplast DNA and morphological classification in east Asian pear (Pyrus spp) [J].
Iketani, H ;
Manabe, T ;
Matsuta, N ;
Akihama, T ;
Hayashi, T .
GENETIC RESOURCES AND CROP EVOLUTION, 1998, 45 (06) :533-539
[10]  
IKETANI H, 1993, GAMMA FIELD S, V32, P63