Methylidene-imidazolone: a novel electrophile for substrate activation

被引：54

作者：

Poppe, L ^{[1
]}

机构：

[1] Budapest Univ Technol & Econ, Inst Organ Chem, H-1111 Budapest, Hungary

来源：

CURRENT OPINION IN CHEMICAL BIOLOGY | 2001年 / 5卷 / 05期

关键词：

D O I：

10.1016/S1367-5931(00)00253-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The recent three-dimensional structure of histidine ammonialyase revealed that the enzyme contains a 3,5-dihydro-5-methylidene-4H-imidazol-4-one (MIO) ring, which forms autocatalytically from an Ala-Ser143-Gly triad. This novel prosthetic group, which is also present in phenylalanine ammonia-lyase, activates substrates by electrophilic interaction. Modern analytical methods, theoretical calculations and molecular biology tools have given further insight into the mode of action of MIO.

引用

页码：512 / 524

页数：13

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