Amyloid formation and disaggregation of α-synuclein and its tandem repeat (α-TR)

被引：30

作者：

Bae, Song Yi ^{[2
]}

Kim, Seulgi ^{[2
]}

Hwang, Heejin ^{[2
]}

Kim, Hyun-Kyung ^{[2
]}

Yoon, Hyun C. ^{[2
]}

Kim, Jae Ho ^{[2
]}

Lee, SangYoon ^{[1
]}

Kim, T. Doohun ^{[2
]}

机构：

[1] Ajou Univ, Chron Inflammatory Dis Res Ctr, Sch Med, Suwon 443749, South Korea

[2] Ajou Univ, Dept Mol Sci & Technol, Grad Sch Interdisciplinary Programs, Suwon 443749, South Korea

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2010年 / 400卷 / 04期

关键词：

alpha-Synuclein; Amyloid fibrils; Parkinson's disease (PD); Ionic liquids (ILs); AGGREGATION;

D O I：

10.1016/j.bbrc.2010.08.088

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

The aggregation of alpha-synuclein is clearly related to the pathogenesis of Parkinson's disease. Therefore, detailed understanding of the mechanism of fibril formation is highly valuable for the development of clinical treatment and also of the diagnostic tools. Here, we have investigated the interaction of alpha-synuclein with ionic liquids by using several biochemical techniques including Thioflavin T assays and transmission electron microscopy (TEM). Our data shows a rapid formation of a-synuclein amyloid fibrils was stimulated by 1-butyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide [BIMbF(3)Im], and these fibrils could be disaggregated by polyphenols such as epigallocatechin gallate (EGCG) and baicalein. Furthermore, the effect of [BIMbF(3)Im] on the alpha-synuclein tandem repeat (alpha-TR) in the aggregation process was studied. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：531 / 536

页数：6

共 29 条

[1]

EGCG remodels mature α-synuclein and amyloid-β fibrils and reduces cellular toxicity [J].

Bieschke, Jan ;

Russ, Jenny ;

Friedrich, Ralf P. ;

Ehrnhoefer, Dagmar E. ;

Wobst, Heike ;

Neugebauer, Katja ;

Wanker, Erich E. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2010, 107 (17) :7710-7715

[2]

Structural insights on physiological functions and pathological effects of α-synuclein [J].

Bisaglia, Marco ;

Mammi, Stefano ;

Bubacco, Luigi .

FASEB JOURNAL, 2009, 23 (02) :329-340

[3]

α-Synuclein aggregation [J].

Bodles, AM ;

Irvine, GB .

PROTEIN AND PEPTIDE LETTERS, 2004, 11 (03) :271-279

[4]

Formation and dissolution of hen egg white lysozyme amyloid fibrils in protic ionic liquids [J].

Byrne, Nolene ;

Angell, C. Austen .

CHEMICAL COMMUNICATIONS, 2009, (09) :1046-1048

[5]

Patterns of protein unfolding and protein aggregation in ionic liquids [J].

Constatinescu, Diana ;

Herrmann, Christian ;

Weingaertner, Hermann .

PHYSICAL CHEMISTRY CHEMICAL PHYSICS, 2010, 12 (08) :1756-1763

[6]

Accelerated in vitro fibril formation by a mutant α-synuclein linked to early-onset Parkinson disease [J].

Conway, KA ;

Harper, JD ;

Lansbury, PT .

NATURE MEDICINE, 1998, 4 (11) :1318-1320

[7]

Amyloid-β fibrillogenesis: Structural insight and therapeutic intervention [J].

DaSilva, Kevin A. ;

Shaw, James E. ;

McLaurin, JoAnne .

EXPERIMENTAL NEUROLOGY, 2010, 223 (02) :311-321

[8]

EGCG redirects amyloidogenic polypeptides into unstructured, off-pathway oligomers [J].

Ehrnhoefer, Dagmar E. ;

Bieschke, Jan ;

Boeddrich, Annett ;

Herbst, Martin ;

Masino, Laura ;

Lurz, Rudi ;

Engemann, Sabine ;

Pastore, Annalisa ;

Wanker, Erich E. .

NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2008, 15 (06) :558-566

[9]

α-Synuclein is part of a diverse and highly conserved interaction network that includes PARK9 and manganese toxicity [J].

Gitler, Aaron D. ;

Chesi, Alessandra ;

Geddie, Melissa L. ;

Strathearn, Katherine E. ;

Hamamichi, Shusei ;

Hill, Kathryn J. ;

Caldwell, Kim A. ;

Caldwell, Guy A. ;

Cooper, Antony A. ;

Rochet, Jean-Christophe ;

Lindquist, Susan .

NATURE GENETICS, 2009, 41 (03) :308-315

[10]

Structural Characteristics of α-Synuclein Oligomers Stabilized by the Flavonoid Baicalein [J].

Hong, Dong-Pyo ;

Fink, Anthony L. ;

Uversky, Vladimir N. .

JOURNAL OF MOLECULAR BIOLOGY, 2008, 383 (01) :214-223

← 1 2 3 →