Polymerization of the actin-like protein MamK, which is associated with magnetosomes

被引：39

作者：

Taoka, Azuma

Asada, Ryuji

Wu, Long-Fei

Fukumori, Yoshihiro ^{[1
]}

机构：

[1] Kanazawa Univ, Dept Life Sci, Grad Sch Nat Sci & Technol, Kakuma, Kanazawa 9201192, Japan

[2] CNRS, IBSM, Chim Bacterienne Lab, UPR9043, F-13402 Marseille 20, France

来源：

JOURNAL OF BACTERIOLOGY | 2007年 / 189卷 / 23期

关键词：

D O I：

10.1128/JB.00899-07

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The recombinant actin-like protein MamK was purified from Escherichia coli and used as an antigen to generate the anti-MamK antibody. Immunostaining studies showed a linear distribution of MamK in Magnetospirillum magnetotacticum cells and of MamK in association with magnetosomes. Moreover, we demonstrated that MamK polymerizes into filamentous bundles in vitro.

引用

收藏

页码：8737 / 8740

页数：4

相关论文

共 20 条

[1]

[Anonymous], 1983, COLD SPRING HARBOR L

[2] Magnetosome formation in prokaryotes [J].

Bazylinski, DA ;

Frankel, RB .

NATURE REVIEWS MICROBIOLOGY, 2004, 2 (03) :217-230

[3] ISOLATION AND PURE CULTURE OF A FRESHWATER MAGNETIC SPIRILLUM IN CHEMICALLY DEFINED MEDIUM [J].

BLAKEMORE, RP ;

MARATEA, D ;

WOLFE, RS .

JOURNAL OF BACTERIOLOGY, 1979, 140 (02) :720-729

[4] The bacterial actin-like cytoskeleton [J].

Carballido-Lopez, Rut .

MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, 2006, 70 (04) :888-+

[5] The assembly of MreB, a prokaryotic homolog of actin [J].

Esue, O ;

Cordero, M ;

Wirtz, D ;

Tseng, Y .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2005, 280 (04) :2628-2635

[6] MreB, the cell shape-determining bacterial actin homologue, co-ordinates cell wall morphogenesis in Caulobacter crescentus [J].

Figge, RM ;

Divakaruni, AV ;

Gober, JW .

MOLECULAR MICROBIOLOGY, 2004, 51 (05) :1321-1332

[7] Magnetosomes are cell membrane invaginations organized by the actin-like protein MamK [J].

Komeili, A ;

Li, Z ;

Newman, DK ;

Jensen, GJ .

SCIENCE, 2006, 311 (5758) :242-245

[8] Molecular mechanisms of magnetosome formation [J].

Komelli, Arash .

ANNUAL REVIEW OF BIOCHEMISTRY, 2007, 76 :351-366

[9] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[10] Deep-etching electron microscopy of cells of Magnetospirillum magnetotacticum:: Evidence for filamentous structures connecting the magnetosome chain to the cell surface [J].

Martins, Juliana Lopes ;

Keim, Carolina Neumann ;

Farina, Marcos ;

Kachar, Bechara ;

Lins, Ulysses .

CURRENT MICROBIOLOGY, 2007, 54 (01) :1-4