Transformation and functional expression of the rFCA-RRM2 gene in rice

被引:12
作者
Attia, K [1 ]
Li, KG [1 ]
Wei, C [1 ]
He, GM [1 ]
Su, W [1 ]
Yang, JS [1 ]
机构
[1] Fudan Univ, Sch Life Sci, Inst Genet, Shanghai 200433, Peoples R China
关键词
FCA gene; gene transformation; heading date; rice;
D O I
10.1111/j.1744-7909.2005.00115.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The primary aim of the present study was to investigate the overexpression of the rice (Oryza sativa L. subsp. japonica var. Zhonghua 11) flowering control gene (rFCA-RRM2) in monocotyledonous model rice. Constitutive expression of rFCA-RRM2 from the Act 1-5 rice promoter caused late flowering in transgenic rice and increased grain weight that was more than 50% higher than that of control plants, which is the first demonstration of rFCA-RRM2 being able to increase rice production. Late flowering was accompanied by strong phenotype and some morphological modifications. These observations suggest that rFCA-RRM2 is a useful tool for phenotype improvement and yield enhancement in cereal crops.
引用
收藏
页码:823 / 830
页数:8
相关论文
共 24 条
[1]   Flowering on time:: genes that regulate the floral transition -: Workshop on the molecular basis of flowering time control [J].
Blázquez, M ;
Koornneef, M ;
Putterill, J .
EMBO REPORTS, 2001, 2 (12) :1078-1082
[2]  
BUGOS RC, 1995, BIOTECHNIQUES, V19, P734
[3]   A protocol for consistent, large scale production of fertile transgenic rice plants [J].
Chen, L ;
Zhang, S ;
Beachy, RN ;
Fauquet, CM .
PLANT CELL REPORTS, 1998, 18 (1-2) :25-31
[4]  
DU X, 2005, IN PRESS DNA SEQUENC
[5]   Structural basis for recognition of the tra mRNA precursor by the sex-lethal protein [J].
Handa, N ;
Nureki, O ;
Kurimoto, K ;
Kim, I ;
Sakamoto, H ;
Shimura, Y ;
Muto, Y ;
Yokoyama, S .
NATURE, 1999, 398 (6728) :579-585
[6]   Identification of class B and class C floral organ identity genes from rice plants [J].
Kang, HG ;
Jeon, JS ;
Lee, S ;
An, GH .
PLANT MOLECULAR BIOLOGY, 1998, 38 (06) :1021-1029
[7]   A GENETIC AND PHYSIOLOGICAL ANALYSIS OF LATE FLOWERING MUTANTS IN ARABIDOPSIS-THALIANA [J].
KOORNNEEF, M ;
HANHART, CJ ;
VANDERVEEN, JH .
MOLECULAR & GENERAL GENETICS, 1991, 229 (01) :57-66
[8]   Genetic control of flowering time in arabidopsis [J].
Koornneef, M ;
Alonso-Blanco, C ;
Peeters, AJM ;
Soppe, W .
ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 1998, 49 :345-370
[9]   Comparative genetics of flowering time [J].
Laurie, DA .
PLANT MOLECULAR BIOLOGY, 1997, 35 (1-2) :167-177
[10]   The transition to flowering [J].
Levy, YY ;
Dean, C .
PLANT CELL, 1998, 10 (12) :1973-1989