Amplified Detection of DNA through an Autocatalytic and Catabolic DNAzyme-Mediated Process

被引：181

作者：

Wang, Fuan ^{[1
]}

Elbaz, Johann ^{[1
]}

Teller, Carsten ^{[1
]}

Willner, Itamar ^{[1
]}

机构：

[1] Hebrew Univ Jerusalem, Inst Chem, IL-91904 Jerusalem, Israel

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2011年 / 50卷 / 01期

基金：

以色列科学基金会;

关键词：

autocatalysis; DNA; DNAzymes; fluorescence; sensors; NUCLEIC-ACID APTAMERS; ELECTROCHEMICAL DETECTION; ENZYME; SENSORS; NANOPARTICLES; HYBRIDIZATION; BIOSENSORS; MUTATIONS; MNAZYMES; BEACONS;

D O I：

10.1002/anie.201005246

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Sense and sensibility: Amplified DNA detection is accomplished by the autocatalytic cleavage of a programmed nucleic acid structure based on the Mg2+-dependent DNAzyme E6 (see picture). The analyte sequence is autocatalytically generated by the DNAzyme from a reporter nucleic acid, which is labeled with a fluorophore and quencher at its two ends. Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：295 / 299

页数：5

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