Growth of various intestinal bacteria on alternansucrase-derived oligosaccharides

被引:49
作者
Holt, SM
Miller-Fosmore, CM
Côté, GL
机构
[1] Western Illinois Univ, Dept Biol Sci, Macomb, IL 61455 USA
[2] USDA ARS, Natl Ctr Agr Utilizat Res, Fermentat Biotechnol Res Unit, Peoria, IL 61604 USA
关键词
alternansucrase; Bifidobacterium; glucooligosaccharides; oligosaccharides; prebiotic;
D O I
10.1111/j.1472-765X.2005.01681.x
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Aims: To determine whether alternansucrase (ASR)-derived oligosaccharides can support the in vitro growth of various intestinal bacteria. Methods and Results: Growth was assessed from each culture after incubation in a medium containing ASR-derived oligosaccharide as sole carbohydrate source. Most of the Bifidobacterium spp. tested showed growth on all five of the oligosaccharides tested while the Lactobacillus spp., Bacteroides thetaiotaomicron, coliforms and pathogenic bacteria displayed no or little growth. Conclusions: The ASR-derived oligosaccharides were selectively utilized by many of the Bifidobacterium spp. tested but did not support significant growth of the Lactobacillus spp., Bact. thetaiotaomicron, coliforms and pathogenic bacteria tested. Significance and Impact of the Study: Alternansucrase-derived oligosaccharides are a potential source of new prebiotics.
引用
收藏
页码:385 / 390
页数:6
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