Optimized tuning of TALEN specificity using non-conventional RVDs

被引：29

作者：

Juillerat, Alexandre ^{[1
]}

Pessereau, Coline ^{[1
]}

Dubois, Gwendoline ^{[1
]}

Guyot, Valerie ^{[1
]}

Marechal, Alan ^{[1
]}

Valton, Julien ^{[1
]}

Daboussi, Fayza ^{[1
]}

Poirot, Laurent ^{[1
]}

Duclert, Aymeric ^{[1
]}

Duchateau, Philippe ^{[1
]}

机构：

[1] CELLECTIS SA, F-75013 Paris, France

来源：

SCIENTIFIC REPORTS | 2015年 / 5卷

关键词：

ACTIVATOR-LIKE EFFECTORS; DNA; TRANSCRIPTION; EFFICIENT; RECOGNITION; NUCLEASES; CRISPR/CAS9; GENES;

D O I：

10.1038/srep08150

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A key feature when designing DNA targeting tools and especially nucleases is specificity. The ability to control and tune this important parameter represents an invaluable advance to the development of such molecular scissors. Here, we identified and characterized new non-conventional RVDs (ncRVDs) that possess novel intrinsic targeting specificity features. We further report a strategy to control TALEN targeting based on the exclusion capacities of ncRVDs (discrimination between different nucleotides). By implementing such ncRVDs, we demonstrated in living cells the possibility to efficiently promote TALEN-mediated processing of a target in the HBB locus and alleviate undesired off-site cleavage. We anticipate that this method can greatly benefit to designer nucleases, especially for therapeutic applications and synthetic biology.

引用

页数：7

共 38 条

[1] Engineering of large numbers of highly specific homing endonucleases that induce recombination on novel DNA targets [J].

Arnould, S ;

Chames, P ;

Perez, C ;

Lacroix, E ;

Duclert, A ;

Epinat, JC ;

Stricher, F ;

Petit, AS ;

Patin, A ;

Guillier, S ;

Rolland, S ;

Prieto, J ;

Blanco, FJ ;

Bravo, J ;

Montoya, G ;

Serrano, L ;

Duchateau, P ;

Pâques, F .

JOURNAL OF MOLECULAR BIOLOGY, 2006, 355 (03) :443-458

[2] Compact designer TALENs for efficient genome engineering [J].

Beurdeley, Marine ;

Bietz, Fabian ;

Li, Jin ;

Thomas, Severine ;

Stoddard, Thomas ;

Juillerat, Alexandre ;

Zhang, Feng ;

Voytas, Daniel F. ;

Duchateau, Philippe ;

Silva, George H. .

NATURE COMMUNICATIONS, 2013, 4

[3] Breaking the Code of DNA Binding Specificity of TAL-Type III Effectors [J].

Boch, Jens ;

Scholze, Heidi ;

Schornack, Sebastian ;

Landgraf, Angelika ;

Hahn, Simone ;

Kay, Sabine ;

Lahaye, Thomas ;

Nickstadt, Anja ;

Bonas, Ulla .

SCIENCE, 2009, 326 (5959) :1509-1512

[4] megaTALs: a rare-cleaving nuclease architecture for therapeutic genome engineering [J].

Boissel, Sandrine ;

Jarjour, Jordan ;

Astrakhan, Alexander ;

Adey, Andrew ;

Gouble, Agnes ;

Duchateau, Philippe ;

Shendure, Jay ;

Stoddard, Barry L. ;

Certo, Michael T. ;

Baker, David ;

Scharenberg, Andrew M. .

NUCLEIC ACIDS RESEARCH, 2014, 42 (04) :2591-2601

[5] Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting [J].

Cermak, Tomas ;

Doyle, Erin L. ;

Christian, Michelle ;

Wang, Li ;

Zhang, Yong ;

Schmidt, Clarice ;

Baller, Joshua A. ;

Somia, Nikunj V. ;

Bogdanove, Adam J. ;

Voytas, Daniel F. .

NUCLEIC ACIDS RESEARCH, 2011, 39 (12) :e82

[6] Targeting G with TAL Effectors: A Comparison of Activities of TALENs Constructed with NN and NK Repeat Variable Di-Residues [J].

Christian, Michelle L. ;

Demorest, Zachary L. ;

Starker, Colby G. ;

Osborn, Mark J. ;

Nyquist, Michael D. ;

Zhang, Yong ;

Carlson, Daniel F. ;

Bradley, Philip ;

Bogdanove, Adam J. ;

Voytas, Daniel F. .

PLOS ONE, 2012, 7 (09)

[7] Comprehensive interrogation of natural TALE DNA-binding modules and transcriptional repressor domains [J].

Cong, Le ;

Zhou, Ruhong ;

Kuo, Yu-chi ;

Cunniff, Margaret ;

Zhang, Feng .

NATURE COMMUNICATIONS, 2012, 3

[8] Genome engineering empowers the diatom Phaeodactylum tricornutum for biotechnology [J].

Daboussi, Fayza ;

Leduc, Sophie ;

Marechal, Alan ;

Dubois, Gwendoline ;

Guyot, Valerie ;

Perez-Michaut, Christophe ;

Amato, Alberto ;

Falciatore, Angela ;

Juillerat, Alexandre ;

Beurdeley, Marine ;

Voytas, Daniel F. ;

Cavarec, Laurent ;

Duchateau, Philippe .

NATURE COMMUNICATIONS, 2014, 5

[9] Chromosomal context and epigenetic mechanisms control the efficacy of genome editing by rare-cutting designer endonucleases [J].

Daboussi, Fayza ;

Zaslavskiy, Mikhail ;

Poirot, Laurent ;

Loperfido, Mariana ;

Gouble, Agnes ;

Guyot, Valerie ;

Leduc, Sophie ;

Galetto, Roman ;

Grizot, Sylvestre ;

Oficjalska, Danusia ;

Perez, Christophe ;

Delacote, Fabien ;

Dupuy, Aurelie ;

Chion-Sotinel, Isabelle ;

Le Clerre, Diane ;

Lebuhotel, Celine ;

Danos, Olivier ;

Lemaire, Frederic ;

Oussedik, Kahina ;

Cedrone, Frederic ;

Epinat, Jean-Charles ;

Smith, Julianne ;

Yanez-Munoz, Rafael J. ;

Dickson, George ;

Popplewell, Linda ;

Koo, Taeyoung ;

VandenDriessche, Thierry ;

Chuah, Marinee K. ;

Duclert, Aymeric ;

Duchateau, Philippe ;

Paques, Frederic .

NUCLEIC ACIDS RESEARCH, 2012, 40 (13) :6367-6379

[10] Structural Basis for Sequence-Specific Recognition of DNA by TAL Effectors [J].

Deng, Dong ;

Yan, Chuangye ;

Pan, Xiaojing ;

Mahfouz, Magdy ;

Wang, Jiawei ;

Zhu, Jian-Kang ;

Shi, Yigong ;

Yan, Nieng .

SCIENCE, 2012, 335 (6069) :720-723

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