The major open reading frame of the β2.7 transcript of human cytomegalovirus:: In vitro expression of a protein posttranscriptionally regulated by the 5′ region

被引：9

作者：

Bergamini, G ^{[1
]}

Reschke, M ^{[1
]}

Battista, MC ^{[1
]}

Boccuni, MC ^{[1
]}

Campanini, F ^{[1
]}

Ripalti, A ^{[1
]}

Landini, MP ^{[1
]}

机构：

[1] Univ Bologna, St Orsola Hosp, Div Microbiol, Dept Clin & Expt Med, I-40138 Bologna, Italy

来源：

JOURNAL OF VIROLOGY | 1998年 / 72卷 / 10期

关键词：

D O I：

10.1128/JVI.72.10.8425-8429.1998

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

beta 2.7 is the major early transcript produced during human cytomegalovirus infection. This abundantly expressed RNA is polysome associated, but no protein product has ever been detected. In this study, a stable peptide of 24 kDa,vas produced in vitro from the major open reading frame (ORF), TRL4. Following transient transfection, the intracellular localization was nucleolar and the expression was posttranscriptionally inhibited by the 5' sequence of the transcript, which harbors two short upstream ORFs.

引用

页码：8425 / 8429

页数：5

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