Enzyme-catalyzed saccharification of model celluloses in the presence of lignacious residues

被引:34
作者
Meunier-Goddik, L [1 ]
Penner, MH [1 ]
机构
[1] Oregon State Univ, Dept Food Sci & Technol, Corvallis, OR 97331 USA
关键词
cellulose; saccharification; cellulase; lignocellulose; switchgrass; lignin;
D O I
10.1021/jf980407b
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
Experiments were designed to determine the relevance of enzyme partitioning, between the cellulose and non-cellulose components of pretreated biomass, with respect to rates of cellulose saccharification in a typical biomass-to-ethanol process;The experimental system included three cellulose preparations (differing in physicochemical properties): a representative Lignin-rich noncellulosic residue (prepared from dilute acid-pretreated switchgrass), an acid-extracted lignin preparation, and a complete Trichoderma reesei cellulase preparation. Enzyme-reactor conditions were typical of those commonly used in biomass-to-ethanol studies. The results were found to be dependent on both the lignin and cellulose preparations used. The noncellulosic lignacious residue, when supplemented at up to 40% (w/w) in cellulose-cellulase reaction mixtures, had little effect on rates and extents of cellulose saccharification. Overall, the results suggest that enzyme partitioning between cellulose and the noncellulosic component of a pretreated feedstock is not likely to have a major impact on cellulose saccharification in typical biomass-to-ethanol processes.
引用
收藏
页码:346 / 351
页数:6
相关论文
共 52 条
[1]  
BAKER AJ, 1973, J ANIM SCI, V36, P768
[2]   COMBINED CHEMICAL AND ENZYMATIC TREATMENTS OF CORN HUSK LIGNOCELLULOSICS [J].
BARL, B ;
BILIADERIS, CG ;
MURRAY, ED ;
MACGREGOR, AW .
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, 1991, 56 (02) :195-214
[3]   ADSORPTION OF CLOSTRIDIUM-THERMOCELLUM CELLULASES ONTO PRETREATED MIXED HARDWOOD, AVICEL, AND LIGNIN [J].
BERNARDEZ, TD ;
LYFORD, K ;
HOGSETT, DA ;
LYND, LR .
BIOTECHNOLOGY AND BIOENGINEERING, 1993, 42 (07) :899-907
[4]  
BRASH JL, 1995, ACS S SERIES, V602
[5]   COMPARATIVE STUDIES ON CELLULOLYTIC ENZYME LIGNIN AND MILLED WOOD LIGNIN OF SWEETGUM AND SPRUCE [J].
CHANG, HM ;
COWLING, EB ;
BROWN, W ;
ADLER, E ;
MIKSCHE, G .
HOLZFORSCHUNG, 1975, 29 (05) :153-159
[6]   ADSORPTION OF HIGH-PURITY ENDO-1,4-BETA-GLUCANASES FROM TRICHODERMA-REESEI ON COMPONENTS OF LIGNOCELLULOSIC MATERIALS - CELLULOSE, LIGNIN, AND XYLAN [J].
CHERNOGLAZOV, VM ;
ERMOLOVA, OV ;
KLYOSOV, AA .
ENZYME AND MICROBIAL TECHNOLOGY, 1988, 10 (08) :503-507
[7]   KINETICS OF ENZYMATIC-HYDROLYSIS OF LIGNOCELLULOSIC MATERIALS BASED ON SURFACE-AREA OF CELLULOSE ACCESSIBLE TO ENZYME AND ENZYME ADSORPTION ON LIGNIN AND CELLULOSE [J].
CONVERSE, AO ;
OOSHIMA, H ;
BURNS, DS .
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 1990, 24-5 :67-73
[8]   ENZYMATIC-HYDROLYSIS OF CELLULOSE - AN OVERVIEW [J].
COUGHLAN, MP .
BIORESOURCE TECHNOLOGY, 1992, 39 (02) :107-115
[9]  
Ehrman T, 1994, STANDARD METHOD ASH
[10]  
EHRMAN T, 1992, 2 STAGE SULFURIC ACI