Electrophoresis in capillary cells with detection gap

被引：2

作者：

Gavrilov, DN ^{[1
]}

Kosobokova, O ^{[1
]}

Khozikov, V ^{[1
]}

Stepukhovitch, A ^{[1
]}

Gorfinkel, V ^{[1
]}

机构：

[1] SUNY Stony Brook, Dept Elect & Comp Engn, Stony Brook, NY 11794 USA

来源：

ELECTROPHORESIS | 2005年 / 26卷 / 18期

关键词：

capillary electrophoresis; field flow; multicapillary array;

D O I：

10.1002/elps.200500237

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel design of the detection zone in multicapillary arrays used for electrophoretic separation is presented. The use of a detection gap (DG), in which the reflective surfaces separating the channels of the array are eliminated, is proposed to improve the illumination and detection of the separated DNA fragments. The electric field compression in the DG is achieved by optimization of the gap geometry. The results of the computer simulation and experiment demonstrate no substantial band-broadening in the DG. We believe that the proposed method will be useful for application in the microfabricated devices.

引用

页码：3430 / 3437

页数：8

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