A capillary array gel electrophoresis system using multiple laser focusing for DNA sequencing

被引：67

作者：

Anazawa, T

Takahashi, S

Kambara, H

机构：

[1] Central Research Laboratory, Hitachi Ltd., Kokubunji

来源：

ANALYTICAL CHEMISTRY | 1996年 / 68卷 / 15期

关键词：

D O I：

10.1021/ac9601831

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A very simple and highly sensitive capillary array gel electrophoresis system is constructed to analyze DNA fragments. On-column detection of DNA migration in a large number of gel-filled capillaries is carried out using side-entry laser irradiation and with a CCD camera, although it has been considered impossible because the irradiation laser is scattered strongly at the surfaces of the first few capillaries. By optimizing optical conditions, the laser beam can be focused repeatedly to irradiate all the capillaries held on a plate by working each capillary as a cylindrical convex lens. DNA sequencing samples migrating in 24 capillaries can simultaneously be analyzed with the system.

引用

页码：2699 / 2704

页数：6

共 29 条

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