Lack of an HSP70 heat shock response in two Antarctic marine invertebrates

被引：57

作者：

Clark, Melody S. ^{[1
]}

Fraser, Keiron P. P. ^{[1
]}

Peck, Lloyd S. ^{[1
]}

机构：

[1] British Antarctic Survey, NERC, Cambridge CB3 0ET, England

来源：

POLAR BIOLOGY | 2008年 / 31卷 / 09期

基金：

英国自然环境研究理事会;

关键词：

Antarctic; climate change; biomarker; stress; heat shock proteins;

D O I：

10.1007/s00300-008-0447-7

中图分类号：

X176 [生物多样性保护];

学科分类号：

090705 ;

摘要：

Members of the HSP70 gene family comprising the inducible (HSP70) genes and GRP78 (glucose-regulated protein 78 kDa) were identified in an Antarctic sea star (Odontaster validus) and an Antarctic gammarid (Paraceradocus gibber). These genes were surveyed for expression levels via Q-PCR after an acute 2-hour heat shock experiment in both animals and a time course assay in O. validus. No significant up-regulation was detected for any of the genes in either of the animals during the acute heat shock. The time course experiment in O. validus produced slightly different results with an initial down regulation in these genes at 2 degrees C, but no significant up-regulation of the genes either at 2 or 6 degrees C. Therefore, the classical heat shock response is absent in both species. The data is discussed in the context of the organisms' thermal tolerance and the applicability of HSP70 to monitor thermal stress in Antarctic marine organisms.

引用

页码：1059 / 1065

页数：7

共 42 条

[1] Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].

Altschul, SF ;

Madden, TL ;

Schaffer, AA ;

Zhang, JH ;

Zhang, Z ;

Miller, W ;

Lipman, DJ .

NUCLEIC ACIDS RESEARCH, 1997, 25 (17) :3389-3402

[2] The SWISS-PROT protein knowledgebase and its supplement TrEMBL in 2003 [J].

Boeckmann, B ;

Bairoch, A ;

Apweiler, R ;

Blatter, MC ;

Estreicher, A ;

Gasteiger, E ;

Martin, MJ ;

Michoud, K ;

O'Donovan, C ;

Phan, I ;

Pilbout, S ;

Schneider, M .

NUCLEIC ACIDS RESEARCH, 2003, 31 (01) :365-370

[3] THERMOTOLERANCE AND SYNTHESIS OF HEAT-SHOCK PROTEINS - THESE RESPONSES ARE PRESENT IN HYDRA-ATTENUATA BUT ABSENT IN HYDRA-OLIGACTIS [J].

BOSCH, TCG ;

KRYLOW, SM ;

BODE, HR ;

STEELE, RE .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (21) :7927-7931

[4] Expression of 70 kDa heat shock proteins in antarctic and New Zealand notothenioid fish [J].

Carpenter, CM ;

Hofmann, GE .

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR AND INTEGRATIVE PHYSIOLOGY, 2000, 125 (02) :229-238

[5] Characterisation of the warm acclimated protein gene (wap65) in the Antarctic plunderfish (Harpagifer antarcticus) [J].

Clark, Melody S. ;

Burns, Gavin .

DNA SEQUENCE, 2008, 19 (01) :50-55

[6] Antarctic marine molluscs do have an HSP70 heat shock response [J].

Clark, Melody S. ;

Fraser, Keiron P. P. ;

Peck, Lloyd S. .

CELL STRESS & CHAPERONES, 2008, 13 (01) :39-49

[7] Low heat shock thresholds in wild Antarctic inter-tidal limpets (Nacella concinna) [J].

Clark, Melody S. ;

Geissler, Paul ;

Waller, Catherine ;

Fraser, Keiron P. P. ;

Barnes, David K. A. ;

Peck, Lloyd S. .

CELL STRESS & CHAPERONES, 2008, 13 (01) :51-58

[8] The HSP70 heat shock response in the Antarctic fish Harpagifer antarcticus [J].

Clark, Melody S. ;

Fraser, Keiron P. P. ;

Burns, Gavin ;

Peck, Lloyd S. .

POLAR BIOLOGY, 2008, 31 (02) :171-180

[9]

COLEMAN CO, 1989, POLAR BIOL, V10, P43

[10] Base-calling of automated sequencer traces using phred.: II.: Error probabilities [J].

Ewing, B ;

Green, P .

GENOME RESEARCH, 1998, 8 (03) :186-194

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